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Spermine has four nitrogen in it. Four nitrogen are helpfull to stabilize positive ions from acid. Electron clod on nitrogen takes part actively in order to stabilize positive ions.

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Role of EDTA in lysis buffer?

EDTA in lysis buffer helps to chelate divalent cations (such as Mg2+ and Ca2+) which are cofactors for nucleases, preventing degradation of nucleic acids. This helps to preserve the integrity of RNA and DNA during the lysis process.


What is the role of glycerol in the lysis buffer?

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What is the role of urea in lysis buffer?

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When isolating DNA from blood, white blood cells (WBC's) are the target. This is because RBC's do not contain a nucleus and therefore do not contain DNA. The function of the lysis buffer is to help in the lysis (or breaking) of white blood cells. WBC's must first be lysed so that the DNA may be released from inside the cell.


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Role of tritonx100 in protein isolation?

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Role of Cl in TE buffer solution?

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