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Role of chloroform in RNA isolation?
it solubilize the lipids and protein and remove them.
Role of beta-mecaptoethanol in DNA isolation?
beta- merceptoethanol denatures the protein by breaking the sulphur bridges in it.
What is the role of Triton in DNA isolation?
it is non-ionic detergent.so it act as non-denaturing agent and membrane protein are not denature.
Role of edta in protein isolation?
Ethylene diamine tetraacetic acid (EDTA) is used in protein isolation to chelate and bind divalent metal ions, such as calcium and magnesium, which could potentially degrade the protein structure and function. By sequestering these metal ions, EDTA helps to stabilize the protein structure during the isolation process, preventing protein denaturation and maintaining its biological activity. Additionally, EDTA can also inhibit metal-dependent proteases, further protecting the integrity of the isolated proteins.
What is the function of tritonx100 in DNA extraction?
Triton X-100 is used as a lysis buffer for DNA separation.
What is the role of NaCl in RNA isolation?
The role of NaCl or sodium chloride in RNA isolation is part of the denaturing process. It is often called the wash step.
What distinguish a protein isolation from the isolation of small oraganic molecule?
The isolation (or extraction) of proteins requires a different method that those for organic molecules. Proteins are extremely sensitive to temperature and ionic conditions. Therefore, the protocol for the isolation of a protein should take into consideration the preparation of the correct buffers and experimental methods to keep the protein intact so it does not degrade during the extraction process. Protein are typically extracted from live cells so the isolation protocol should include disrupting the cell and removing the protein undamaged. With organic molecules however, liquid-liquid extraction or 2-phase extraction may be carried out (depending on the properties of the organic substance)
Role of CTAB in DNA isolation?
It sequester carbohydrates in the solution
What is the role of rRNA the protein synthesis?
it hold the protein synthesis in the ribosomes
What is the role of 2-mercaptoethanol in the isolation of genomic DNA from a plant source?
It is an antioxidant.
What is the role of SE-VEG in DNA isolation?
the role seveg in plant DNA extractions is to remove chlorophyll and similar pigments
What is the center of protein synthesis?
Ribosomes play important role in the protein synthesis.
