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TritonX-100 was used for Remove the SDS-From the crude protein, during homogenization the cell lysis buffer as contain SDS otherwise no need.
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Role of chloroform in RNA isolation?
it solubilize the lipids and protein and remove them.
Role of beta-mecaptoethanol in DNA isolation?
beta- merceptoethanol denatures the protein by breaking the sulphur bridges in it.
What is the role of Triton in DNA isolation?
it is non-ionic detergent.so it act as non-denaturing agent and membrane protein are not denature.
Role of phenol chloroform in DNA isolation?
phenol is used in order to remove protein impurities from the DNA to yield pure dna while chloroform prevents shearing of DNA during isolation.
What is the role of sodium acetate in DNA extraction?
sodiom acetat reaction with membrane protein and cause that persipitate and help to dna isolation
What is the role of NaCl in RNA isolation?
The role of NaCl or sodium chloride in RNA isolation is part of the denaturing process. It is often called the wash step.
What is the function of tritonx100 in DNA extraction?
Triton X-100 is used as a lysis buffer for DNA separation.
What distinguish a protein isolation from the isolation of small oraganic molecule?
The isolation (or extraction) of proteins requires a different method that those for organic molecules. Proteins are extremely sensitive to temperature and ionic conditions. Therefore, the protocol for the isolation of a protein should take into consideration the preparation of the correct buffers and experimental methods to keep the protein intact so it does not degrade during the extraction process. Protein are typically extracted from live cells so the isolation protocol should include disrupting the cell and removing the protein undamaged. With organic molecules however, liquid-liquid extraction or 2-phase extraction may be carried out (depending on the properties of the organic substance)
Role of CTAB in DNA isolation?
It sequester carbohydrates in the solution
What are the function of sodium acetate in dna isolation?
Sodium acetate buffer helps by reacting with the membrane protein and precipitating them, thus facilitating the dna isolation.
Role of sucrose in DNA isolation from human blood?
Sucrose performs the function of osmoregulation in the protocol of DNA isolation from blood
What is role of Cetyl trimethyl ammonium bromide in DNA isolation?
it act as as a cationic detergent for the isolation dna from the given sample
