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What is the use of resuspension solution in DNA isolation?
Resuspension buffer (solution I) is used for the isolation of plasmid DNA by alkaline lysis method. Bacterial cells, obtained from the culture (liquid culture or colonies grown on agar plate), is resuspended in this buffer. The purpose of this buffer is to provide an optimal starting pH (pH 8.0) and an ideal condition for subsequent lysis.
Why use fresh thioglycollate medium?
A fluid thioglycollate is preferably used soon after preparation to minimize the amount of oxygen that is permitted to diffuse towards the bottom of the medium. The longer you wait before using this medium, the more oxygen is allowed to diffuse into it; more oxygen will negatively affect the growth of obligate anaerobes but would positively affect the growth of aerobic organisms.
What medium is use for catalase test?
Hydrogen peroxide is the medium used for the catalase test.
What is the use of inspissator?
Inspissator for use in the preparation of TB culture medium (TBT-T100IN)
What happened to the water after filtering?
After filtering muddy water, the mud is left behind as a sludge. If the filter is not frequently cleaned of this sludge, the filtering efficiency is reduced, even to stop altogether.
What is the use of resuspension solution in DNA isolation?
Resuspension buffer (solution I) is used for the isolation of plasmid DNA by alkaline lysis method. Bacterial cells, obtained from the culture (liquid culture or colonies grown on agar plate), is resuspended in this buffer. The purpose of this buffer is to provide an optimal starting pH (pH 8.0) and an ideal condition for subsequent lysis.
What is a medium size body of fresh water surrounded by land?
A medium size body of fresh water surrounded by land is called a lake. Lakes can vary in size and shape, and are formed by various natural processes such as tectonic activity, glaciation, or volcanic activity. Lakes are important habitats for aquatic plants and animals, and provide natural resources for human use.
Does Medium consists of what a work of art is made?
In art, the medium is what a work of art is made of. I am an oil painter, so my medium is oil paint. Other artists could use acrylic paint as a medium or watercolor as a medium. A sculptor could use stone as a medium. Another would use clay as a medium for making pottery. A carver could use wood as a medium.
What if you use fresh sage in a crock pot?
No problem to use fresh sage or any other fresh herb when cooking in stock pot.
What is a charcoal medium?
That means you use "charcoal" as your medium. Or your material of choice.
Can fresh spinach be frozen for later use?
Yes, fresh spinach can be frozen for later use.
Can you freeze fresh juice for later use?
Yes, you can freeze fresh juice for later use.
How do you use fresh rosemary?
you can use fresh rosemary by making rosemary tea or by putting rosemary on some foods
Can you use fresh sticks ground cinnamon in cookies?
yes you can use one fresh stick of cinnamon in cookies
What can I use as a substitute for fresh coriander in this recipe?
You can use dried coriander or cilantro as a substitute for fresh coriander in the recipe.
What is supernatant solutions?
Supernatant liquid is the upper layer of fluid found after a mixture has been centrifuged. Because of its lower density, the fluid and the components in it have a lesser tendency to migrate to the bottom of a centrifuge tube.
What medium did Perugino use?
fresco
