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How do you calculate the melting temperature of oligonucleotide?
The melting temperature (Tm) of an oligonucleotide can be estimated using formulas like the Wallace rule, nearest-neighbor method, or online tools. These methods take into account factors like oligonucleotide length, GC content, and ionic conditions to predict the Tm based on the sequences' propensity to form stable base pairs. Experimental validation is often necessary to verify the calculated Tm.
What is adapter in recombinant DNA technology?
adapters are smal duble stranded oligonucleotide molecules having a known 3' overhang part which flank the middle double stranded part...when it is phophatase treated it is called modified adapter...rikoganguly@gmail.com
Site directed mutagenesis is a molecular biology method used to make what?
Site directed mutagenesis, also called site-specific mutagenesis or oligonucleotide-directed mutagenesis, is used to make specific, intentional changes to the DNA sequence of a gene and/or gene products. It is used to investigate the structure and biological activity of RNA, DNA, and protein molecules. Also used for protein engineering.
What kind of macromolecule is DNA polymerase?
DNA polymerase is an enzyme that helps catalyze the polymerization of DNA bases (deoxyribonucleotides) into a DNA strand.
What is the principle of allele specific oligonucleotide polymerase chain reaction?
The principle of allele-specific oligonucleotide polymerase chain reaction (ASO-PCR) involves designing primers that are specific to the target allele sequences. By using these specific primers, only the allele of interest is amplified during PCR, allowing for the detection and identification of specific alleles. ASO-PCR is commonly used to genotype individuals based on the presence or absence of specific genetic variations.
How do you calculate the tmfvalue?
The melting temperature TM, characterises the stability of the DNA hybrid formed between an oligonucleotide and its complementary strand. At TM 50% a given oligonucleotide can hybridised to its complementary strand. By: Zoya Mobeen
Can you please provide the CAS No for the oligonucleotide i.e. oligotrimedylic acid?
I believe there is no CAS No for oligonucleotide. However, here's the CAS No for DNA and RNA. Hope it will be helpful. Deoxyribonucleic Acid CAS No. 9007-49-2 Ribonucleic Acid CAS No. 66082-38-0
Which of the following is needed in the process of DNA replication?
The ingredients needed for DNA replication include a short oligonucleotide primer and dNTPS. It also needs DNA polymerase and different transcription and translation factors.
What is dharmacon research about?
Dharmacon was founded to research, develop, and market a new technology for RNA oligonucleotide synthesis. RNA include a large family of biological molecules vital in unlocking DNA.
What is linker in recombinant DNA technology?
In recombinant DNA technology, a linker is a short, double-stranded DNA sequence that contains restriction sites for cloning DNA fragments. Linkers are used to join different DNA fragments together by ligating them into the restriction sites within the linker, allowing for the creation of chimeric DNA molecules.
What oligonucleotide probe would best hybridize with the DNA sequence 5'-AAAAGGTTCC-3'?
3' TTTTCCAAGG 5' for a DNA probe; 3' UUUUCCAAGG 5'for an RNA probe.The reasons:1Paired nucleic acid strands are antiparallel, meaning that they run in opposite directions. So the 5' end of one strand is opposite the 3' end of the other strand.2Hybridizing follows base pairing rules: A pairs with T (in DNA) or U (in RNA), and C pairs with G.
What kind of mixture is DNA?
DNA is not a mixture.
How many kinds of DNA are there?
There is only one kind of DNA
How do you calculate the melting temperature of oligonucleotide?
The melting temperature (Tm) of an oligonucleotide can be estimated using formulas like the Wallace rule, nearest-neighbor method, or online tools. These methods take into account factors like oligonucleotide length, GC content, and ionic conditions to predict the Tm based on the sequences' propensity to form stable base pairs. Experimental validation is often necessary to verify the calculated Tm.
Why are there nucleotides A T G and C in the master mix and what are the other components and what are there functions?
The nucleotides also called dNTPs are free nucleotides that are used in the replication of new strands. The complete master mix contains Taq DNA polymerase, deoxynucleotides, oligonucleotide primers, magnesium ions, and buffer. Function: Taq DNA-polymerase: Enzyme is heat-resistant as it can withstand the highest temperature during the PCR process. deoxynucleotides (dNTPs): Nucleotides that are used in the replication of new strands. Oligonucleotide primers: Pieces of DNA complementary to the template that tell DNA polymerase exactly where to start making copies. Magnesium ions: A cofactor (catalyst) required by DNA polymerase to create the DNA chain. Salt buffer: Provides the optimum ionic environment and pH for the PCR reaction.
What is adapter in recombinant DNA technology?
adapters are smal duble stranded oligonucleotide molecules having a known 3' overhang part which flank the middle double stranded part...when it is phophatase treated it is called modified adapter...rikoganguly@gmail.com
Site directed mutagenesis is a molecular biology method used to make what?
Site directed mutagenesis, also called site-specific mutagenesis or oligonucleotide-directed mutagenesis, is used to make specific, intentional changes to the DNA sequence of a gene and/or gene products. It is used to investigate the structure and biological activity of RNA, DNA, and protein molecules. Also used for protein engineering.
