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1)prepare the blood plate(black background).

2)add one drop serum

3)add antigen (o,H,AH,BH)

4)mix the antigen and serum.

5)rotate the sample (1 min)

6)result:positive reactive

:negative non-reactive

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Name of the blood test for typhoid?

The blood test for typhoid is called the Widal test. It detects antibodies in the blood against the Salmonella typhi bacteria, which causes typhoid fever.


What does typhi h and o mean in widal test?

In a Widal test, "H" refers to the flagella antigen of Salmonella typhi while "O" refers to the somatic antigen of the bacteria. The presence of antibodies against these antigens indicates a recent or current infection with Salmonella typhi, the bacterium that causes typhoid fever.


Why to do serial dilution for WIDAL test?

Serial dilution in Widal test helps to determine the titer of antibodies in the patient's serum by observing the agglutination reaction with specific antigens. By diluting the serum sample in a stepwise manner, the titer at which agglutination occurs can be determined, providing valuable information about the presence and concentration of antibodies against Salmonella typhi and Paratyphi antigens in the blood.


Biochemical test used in identification of salmonella typhi?

The biochemical test commonly used to identify Salmonella typhi is the Widal test. This test detects antibodies produced in response to a Salmonella typhi infection by measuring agglutination (clumping) reactions between the bacteria and specific antibodies in the patient's serum. Other tests like blood culture and polymerase chain reaction (PCR) can also be used for confirmation.


The procedure indicates that the amount of time th test tube was kept in th boiling water bath should be minimized why was this necessary?

The procedure indicates that the amount of time the test tube was kept boiling in the water bath should be minimized. The water bath should never be allowed to boil dry.

Related Questions

What is false positive widal test?

What is false positive in widal test


What is the difference between widal slide test Antigen and widal tube test antigen?

The main difference between the Widal slide test antigen and the Widal tube test antigen lies in the method of reaction visualization. In the Widal slide test, the reaction is observed on a glass slide under a microscope, whereas in the Widal tube test, the reaction is seen in small test tubes. Both tests detect antibodies against specific Salmonella antigens, but differ in their setup and visual interpretation.


Will Widal Test after positive resul after treatment in Typhoid?

I mean widal test result positive after treatment


How do you read widal test?

Isolated Widal test reading does not have much clinical significance. Rising titre of Widal test done one week apart, in a febrile patient, indicates typhoid fever. You can have such rising titre of Widal test in healthy patient, after dose of typhoid vaccine.


What significant titer for widal's test?

1:80 titre is significant for determination of widal positive.


What are the diagnosis for typhoid fever?

widal test


Do antibiotics affect widal test results?

To a certain extent yes.The post antibiotics Widal Test will have Low titers of agglutinins.Hence the test may be reported as False Negative.


Name of the blood test for typhoid?

The blood test for typhoid is called the Widal test. It detects antibodies in the blood against the Salmonella typhi bacteria, which causes typhoid fever.


What is a manual method?

manual test of widal method


How you can confirm that typhoid is eradicated with treatment?

widal test.


How would you distinguish between an incubatory carrier and a chronic carrier of typhoid fever?

Widal test is negative in incubatory carrier. Widal test is always positive in chronic carrier.


Why widal test appears negative in first week of typhoid fever?

In Widal test, you measure the antibodies. It takes minimum one week to develop the antibodies of typhoid bacteria.