When observing a specimen at 400x magnification, it may disappear from view due to a combination of factors, including limited depth of field and the need for precise focus. At higher magnifications, the field of view becomes smaller, making it easy to lose sight of the specimen. Additionally, any slight movement or misalignment of the slide can cause the specimen to move out of the focal plane, resulting in its disappearance. Proper adjustment and steady handling are essential to keep the specimen in view at high magnification.
The diameter of the field of view decreases when changing from low to high power magnification. This is because higher magnification zooms in closer on the specimen, limiting the area of the specimen that can be seen at one time.
Specimen magnification on a microscope is calculated by multiplying the magnification of the eyepiece by the magnification of the objective lens. For example, if the eyepiece magnifies 10 times and the objective lens magnifies 40 times, the total magnification would be 10 x 40 = 400 times.
It depends on what the specimen is. If for example - the specimen is a person's finger-print, then low magnification is sufficient. However - if the specimen is a sample of blood, a higher magnification would be needed to show individual blood cells.
The distance between the objective and the specimen being observed would be the least under high magnification. Higher magnification requires the objective lens to be closer to the specimen in order to achieve detailed resolution.
When the magnification is increased from 10x to 40x, the field of view becomes smaller and the depth of field decreases. This can cause the specimen to go out of focus or move out of the field of view, appearing to disappear. Adjusting the focus and position of the specimen may help bring it back into view at the higher magnification.
Before changing from one magnification to a higher magnification, you should ensure that the current specimen is in focus at the lower magnification. This will help you maintain the clarity and sharpness of the image when transitioning to a higher magnification.
As magnification increases, the field of view decreases. This is because higher magnification enlarges the image being viewed, resulting in a smaller portion of the specimen being visible in the field of view. Conversely, decreasing magnification expands the field of view, allowing more of the specimen to be seen at once.
Increasing magnification changes the field of view, depth of field and amount of detail seen.
The magnification level required for viewing the specimen in detail depends on the size and complexity of the specimen. Typically, a magnification level of 400x to 1000x is needed for detailed viewing of biological specimens.
by the objective lens
When observing a specimen at 400x magnification, it may disappear from view due to a combination of factors, including limited depth of field and the need for precise focus. At higher magnifications, the field of view becomes smaller, making it easy to lose sight of the specimen. Additionally, any slight movement or misalignment of the slide can cause the specimen to move out of the focal plane, resulting in its disappearance. Proper adjustment and steady handling are essential to keep the specimen in view at high magnification.
The diameter of the field of view decreases when changing from low to high power magnification. This is because higher magnification zooms in closer on the specimen, limiting the area of the specimen that can be seen at one time.
Specimen magnification on a microscope is calculated by multiplying the magnification of the eyepiece by the magnification of the objective lens. For example, if the eyepiece magnifies 10 times and the objective lens magnifies 40 times, the total magnification would be 10 x 40 = 400 times.
The magnification of the specimen under low power optics, lpo, is 10 times and the magnification of the specimen under high power optics, hpo, depends on the power of the microscope but is usually at least 500 times or more.
It depends on what the specimen is. If for example - the specimen is a person's finger-print, then low magnification is sufficient. However - if the specimen is a sample of blood, a higher magnification would be needed to show individual blood cells.
You must stain the specimen for the magnification to show them clearly.