A sample should be examined with low power first when observing any sample for the first time under the microscope. Now if you want to focus on one particular region in the sample then you can step it up to high power lens. Objective lens are not that expensive and good way to look at some simple tissue structures.
A magnification of 100x typically requires the use of a 100x objective lens on a microscope. Different microscopes may have varying objective lens magnifications, but for a total magnification of 100x, the 100x objective is commonly used.
The objective lens with the lowest magnification, usually 4x. Be sure to clean all the lens with lens paper before putting the microscope away.
You should start by using the lowest power objective lens, usually the 4x or 10x objective, to locate and center the specimen on the slide. Once the specimen is in focus, you can switch to higher magnifications for more detailed viewing.
If you are using the oil immersion objective on a microscope, you must use oil to increase the resolution of the lens. These lens are used at very high magnification.
scanning objective
You should use the lowest value lens. It is the shortest one.
The fine adjustment knob should be used with high power magnification because it allows for smaller, more precise movements of the objective lens. This helps to prevent damage to the slide and objective lens, as well as minimize the risk of crashing the objective lens into the slide.
To achieve a total magnification of 100x, you would use a 10x ocular lens (eyepiece) with a 10x objective lens. The total magnification is calculated by multiplying the magnification of the ocular lens (10x) by the magnification of the objective lens (10x).
The ocular is the upper lens and objective is the lower lens
low power objective lens
Storing a microscope with the low power objective lens in place helps protect the lens and prevents accidental damage or contamination during storage. The low power lens is less delicate compared to other high-power lenses, making it a safer option for storage to ensure the microscope remains in good condition for future use.
So you do not crack the high power objective lens - this lens is very fragile and expensive.
A magnification of 100x typically requires the use of a 100x objective lens on a microscope. Different microscopes may have varying objective lens magnifications, but for a total magnification of 100x, the 100x objective is commonly used.
So you don't crack the slide or damage the lens
The objective lens with the lowest magnification, usually 4x. Be sure to clean all the lens with lens paper before putting the microscope away.
so the objective lens will not touch the slide.
10x