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DNA ligase
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What cuts DNA into fragments?
cutting of DNA into fragments simply means application of suitable restriction enzyme to it.now a days two types of restriction enzymes are available,1)exonucleases,which cut at end portion of DNA and 2)endonucleases ,which cut at specific inner site.
At the DNA synthesis G2 checkpoint DNA replication is checked by 1 receptor proteins 2 Repair enzymes 3 electron transport chains 4 cell surface markers?
receptor proteins
Why exonucleases are not used in r DNA technology?
Endonucleases are used in biotechnology because they are able to be applied to a specific part of a DNA sequence and will cut the DNA only in these locations. Exonucleases on the other hand have the following issues: 1) An exonuclease will digest and entirely consume DNA/RNA. As opposed to cut a specific location of DNA, they will eat the entire strand if they are not stopped. 2) They will only begin digestion at the ends of the DNA/RNA molecule. Circular plasmid DNA will not be affected by an exonuclease, because there are no beginnings or ends to the DNA sequence. 3) More over, as mentioned in (2) they will only begin digestion at the ends of the DNA sequences, thus they are much less accurate in where they begin cutting the DNA/RNA. Because of this lack of control of exonucleases, they are difficult to use and generally not used in biotechnological techniques.
What Replicates DNA by making a complementary copy of the original?
DNA replicates itself. What happens is, with the help of enzymes, the nitrogenous bases of DNA break away from each other, then copy themselves and re-attach. This process forms 2 strands of identical DNA.
Does mitosis copy the original strand?
Yes ,In mitosis , the original strands split into 2 parts with the help of enzymes forming "Y" shaped split .The exposed ends of the strand ,two new complementary strands are synthesised. So the duplicated strand of DNA contains 50% of the original strand and rest of the 50% is synthesised.
What enzymes help separarte the 2 strands of nucleotides on DNA?
DNA helicase
What cuts DNA into fragments?
cutting of DNA into fragments simply means application of suitable restriction enzyme to it.now a days two types of restriction enzymes are available,1)exonucleases,which cut at end portion of DNA and 2)endonucleases ,which cut at specific inner site.
When greasing the fittings on a car how much do you use?
2-3 pumps into small joints, tie rod ends, u-joints, etc. 3-4 pumps into larger joints, ball joints, etc.
What is it called when DNA separate into two strands?
DNA Replication by enzymes that copy DNA for chromosomes in the new cell after cell division (mitosis)
DNA strands can be clipped crosswise at selected positions by using enzymes called?
DNA can be cut into smaller fragments by enzymes (which are proteins) known as restriction endonucleases (REN's). These enzymes are sequence specific - meaning they produce a cut only at a particular site on the DNA strand. This site where the cut is produced is called the restriction site. Restriction sites are 4 - 6 nucleotides in length. Every restriction enzyme has a different restriction site. This property allows researchers to treat two different DNA samples with the same set of restriction enzymes and then analyze the resulting fragments.A. DNA finger printing
Describe the difference between a single digest and a double digest?
Restriction enzymes (endonucleases) are used for a variety of reasons in molecular genetics, including obtaining a "map" and cloning DNA. Single digests consitute DNA being treated with one restriction endonuclease, whereas double digests contain 2 enzymes. At times, it is difficult (or not possible) to perform double digests ... especially when the 2 enzymes have very different requirements for their activities (e.g. salt concentration, temperature optimums, ...). If a DNA restriction map is known for a particular enzyme, and if the DNA is treated with this enzyme, then one can ascertain whether the digest was complete or not. However, if a restrictioin map is just being compiled, and if the DNA is treated with 2 enzymes in a double digest, at times difficulties may arise in determining the map if either (or both) enzymes did not completely digest the DNA.
What have been present in Earth's living organisms for the least amount of evolutionary time a. photosystems I and II b. genes composed of DNA c. enzymes that catalyze glycolysis?
enzymes that catalyze glycolysis photosystems 1 and 2 cell walls nuclei genes composed of DNA
What is the principle enzyme involved in DNA replication?
Two of the enzymes involved in DNA replication are helicase and DNA polymerase. Helicase unwinds the DNA strand and DNA polymerase makes a copy.
Of what biological significance is the DNA molecule?
The biological significance of a DNA molecule is that it: 1) Controls the synthesis of proteins (as well as enzymes because they are also proteins). 2) Is responsible for carrying hereditary characteristics.
At the DNA synthesis G2 checkpoint DNA replication is checked by 1 receptor proteins 2 Repair enzymes 3 electron transport chains 4 cell surface markers?
receptor proteins
Why exonucleases are not used in r DNA technology?
Endonucleases are used in biotechnology because they are able to be applied to a specific part of a DNA sequence and will cut the DNA only in these locations. Exonucleases on the other hand have the following issues: 1) An exonuclease will digest and entirely consume DNA/RNA. As opposed to cut a specific location of DNA, they will eat the entire strand if they are not stopped. 2) They will only begin digestion at the ends of the DNA/RNA molecule. Circular plasmid DNA will not be affected by an exonuclease, because there are no beginnings or ends to the DNA sequence. 3) More over, as mentioned in (2) they will only begin digestion at the ends of the DNA sequences, thus they are much less accurate in where they begin cutting the DNA/RNA. Because of this lack of control of exonucleases, they are difficult to use and generally not used in biotechnological techniques.
How many grease fittings does a 2001 Chevy 2500 4WD HD have?
Should have 11. Upper and lower ball joints (4), outer tie rod ends (2), inner tie rods ends (2), idler arm (2) and pitman arm (1).
