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What else can I help you with?
What molecules are used to extract the gene of interest and recombine the DNA?
restriction enzyme
Scientist wants to insert a new gene into a bacterium. What is the first step in this process?
Cutting the gene out of DNA with enzymes - gradpoint
What happens when a restriction enzyme is used on DNA?
when restriction enzyme is use on DNA basically it just first losen up the DNA, usally DNA is coiled, and so the restriction enzyme jsut breka the DNA and leave a sticky end, so that it can be put back together, the cell have to be able to do that because in nature, that's the way for cell to stop protein production and the cell still need that gene
Which enzyme would cut the plasmid without disrupting the function of?
Perhaps you mean a restriction enzyme, but not disrupting the function of whatever is not too clear. I think if you cut a plasmid with any restriction enzyme I am familiar with the function of that plasmid would be disrupted.
Why is the bacterium now able to make insulin?
The bacterium has been genetically modified to contain the human insulin gene. This gene allows the bacterium to produce insulin when it is transformed with the gene and given the appropriate conditions for protein synthesis.
What molecules are used to extract the gene of interest and recombine the DNA?
restriction enzyme
Scientist wants to insert a new gene into a bacterium. What is the first step in this process?
Cutting the gene out of DNA with enzymes - gradpoint
What is the first step in inserting a new gene into a bacterium?
The first step in inserting a new gene into a bacterium is to isolate the gene of interest and prepare it for insertion. This can involve cutting the gene with restriction enzymes and ligating it into a vector, which is a piece of DNA that can deliver the gene into the bacterium.
Why must you use an enzyme that will not cut anywhere within the gene that you are inserting into a plasmid?
If you are trying to take a gene from a DNA strand and put insert it into a plasmid, you wouldn't want a restriction enzyme to cut that gene up, or else it would be pretty useless. In other words, you need an enzyme or two that cuts outside that gene so that it can be functional after it's inserted into a plasmid. After your gene of interest is inserted into a plasmid, the plasmid can be put back into a bacterium, then you could genetically engineer plants with it or let the bacterium reproduce and produce many copies of a protein that you had wanted to make in the first place.
How could you use genetic engineering techniques to make transformed bacteria that produce the enzyme?
Extract DNA from the cells of people who can make the digestion enzyme. Cut the DNA with restriction enzymes to cut out the gene that codes for the enzyme. Use gel electrophoresis to locate the gene. Then, use polymerase chain reaction to make copies of the gene. Choose a plasmid that has an antibiotic-resistance genetic marker, and cut the plasmid with the smae restriction enzyme use to cut out the hyman gene. Insert the copies of the human gene into the plasmids. Allow bacterial cells to take in the plasmids. Select for transformed bacteria by growing them in a culture containing the antibiotic. These bacteria will make the digestion enzyme.
Which enzyme would cut the human DNA shown in Part A on both sides of the vgp gene but not inside the gene?
1. Which enzyme(s) would cut the human DNA shown in Part A on both sides of the vgp gene, but not inside the gene? Answer: BamHI, HaeIII, and HindIII 2. Which enzymes(s) would cut the plasmid without disrupting the function of the amp^R gene? Answer: BamHI, EcoRI, and HaeIII 3. Which enzyme(s) would produce sticky ends when cutting both the human DNA and the plasmid? Answer: BamHI, EcoRI, and HindIII 4. Which one restriction enzyme satisfies all three of the requirements listed above? Answer: BamHI only
How you clone a gene using cosmid as a vector?
The first step is restriction of the cosmid and the foreign DNA with the restriction enzyme, then ligating the fragments together. Thereafter, the cosmids are loaded into the phage capsid, which leads to the expression of the foreign gene through transduction.
Why restriction enzyme cannot cut its own DNA?
Restriction enzymes are produced by bacteria to help destroy foreign, invading DNA, such as the DNA of bacteriophage (a virus that infects bacterial cells). Every restriction enzyme comes with a methylase enzyme, or more specifically, a DNA methyltransferase. The methylase enzyme methylates (adds a methyl group) to the restriction endonuclease site on the cell's own DNA, which protects the sites from the restriction enzyme so that it does not degrade its own DNA.
How is the action of a restriction enzyme changes when a mutation occurs in a DNA base sequence?
A restriction enzyme works by locating ends of very shorts strands of nucleotides, so in a way it is impossible to create a mutation that renders a restriction enzyme completely useless. Although if many mutations occur in an important part of a gene the host cell may be saved. The restriction enzyme will not change (unless the gene that created it was mutated itself), but it is nigh impossible to render them useless as they cut 4-6 base pair combinations at a time. If there were a completely alien bacteria that had no combination sequence that fits the enzyme's needs, it would as I said before render them completely useless. For any real change to occur you have to have the gene either evolve or mutate.
How do you isolate the gene in genetic engineering?
You isolate the gene in genetic engineering by first locating the gene you wish to be isolated. Then you use a restrictive enzyme to isolate it, and lastly take the gene out
What is the significance of the restriction enzyme GGATCC in molecular biology research?
The restriction enzyme GGATCC is significant in molecular biology research because it recognizes and cuts DNA at a specific sequence, allowing scientists to manipulate and study DNA molecules. This enzyme is commonly used in genetic engineering techniques such as gene cloning and DNA fingerprinting.
How can one isolate genes effectively using the method of gene isolation?
To isolate genes effectively using the method of gene isolation, researchers typically use techniques such as polymerase chain reaction (PCR) or restriction enzyme digestion to extract and amplify specific DNA sequences. This allows for the targeted isolation of genes from a larger genome, enabling further study and analysis.
