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When to Use dmso or glycerol?
DMSO (dimethyl sulfoxide) is commonly used as a cryoprotectant for freezing cells, while glycerol is often used as a stabilizer for enzymes and proteins. The choice between DMSO and glycerol depends on the specific application and the stability requirements of the biological material being used.
What are the possible impurities in dmso?
Possible impurities in DMSO (dimethyl sulfoxide) can include water, dimethyl sulfide, dimethyl sulfone, acetone, and other organic compounds. These impurities can affect the stability and purity of DMSO, altering its properties and potential uses. Regular purification methods such as distillation or filtration are typically used to remove impurities from DMSO.
What is the role of DMSO in cell culture?
DMSO (dimethyl sulfoxide) is commonly used in cell culture as a cryoprotectant to prevent ice crystal formation and cell damage during freezing and thawing of cells. It helps preserve cell viability and functionality when cells are stored at low temperatures. DMSO is also used as a solvent for some compounds and reagents in cell culture experiments.
Smell after using dmso?
DMSO can have a garlic-like smell when it reacts with compounds on your skin. This effect is temporary and should dissipate once the DMSO is fully absorbed or evaporated. It's important to use DMSO in a well-ventilated area to minimize the smell.
Why is DMSO used in PCR?
DMSO (dimethyl sulfoxide) is used in PCR to facilitate the denaturation of DNA at high temperatures by destabilizing the secondary structure of DNA. It can also help to improve the specificity and yield of PCR reactions by preventing the formation of secondary structures that can inhibit the amplification process. Additionally, DMSO can help reduce the formation of primer dimers and nonspecific amplification products.
Why is DMSO used as a control?
Many test compounds (drugs, inhibitors, etc) are not soluble in water and therefore dimethylsulfoxide (DMSO) is used as a solvent instead. The compound dissolved in DMSO is what is used to treat the cell or animal and therefore you must prove that it is the compound, not the DMSO which is causing any results seen. To ensure this, you have a control that contains only DMSO and not the test compound. This is often referred to as a "vehicle control".
When to Use dmso or glycerol?
DMSO (dimethyl sulfoxide) is commonly used as a cryoprotectant for freezing cells, while glycerol is often used as a stabilizer for enzymes and proteins. The choice between DMSO and glycerol depends on the specific application and the stability requirements of the biological material being used.
What is the difference betwen DMSO and DMSO-d6 solution?
DMSO (dimethyl sulfoxide) is a common organic solvent, whereas DMSO-d6 is a deuterated form of DMSO used in NMR spectroscopy as a solvent. The "d6" indicates that the hydrogen atoms in DMSO have been replaced with deuterium, making it suitable for NMR analysis due to the absence of NMR-active protons.
Is DMSO a protic or aprotic solvent?
DMSO is an aprotic solvent.
Is DMSO a strong nucleophile?
Yes, DMSO is a strong nucleophile.
What are the possible impurities in dmso?
Possible impurities in DMSO (dimethyl sulfoxide) can include water, dimethyl sulfide, dimethyl sulfone, acetone, and other organic compounds. These impurities can affect the stability and purity of DMSO, altering its properties and potential uses. Regular purification methods such as distillation or filtration are typically used to remove impurities from DMSO.
What is the role of DMSO in cell culture?
DMSO (dimethyl sulfoxide) is commonly used in cell culture as a cryoprotectant to prevent ice crystal formation and cell damage during freezing and thawing of cells. It helps preserve cell viability and functionality when cells are stored at low temperatures. DMSO is also used as a solvent for some compounds and reagents in cell culture experiments.
Smell after using dmso?
DMSO can have a garlic-like smell when it reacts with compounds on your skin. This effect is temporary and should dissipate once the DMSO is fully absorbed or evaporated. It's important to use DMSO in a well-ventilated area to minimize the smell.
How to mix DMSO with water effectively?
To mix DMSO with water effectively, slowly add the DMSO to the water while stirring continuously. This will help prevent the DMSO from clumping or separating from the water. It is important to mix the two substances thoroughly to ensure a homogeneous solution.
What is Polands province?
Actually pland does not have a province.
Why is DMSO used in PCR?
DMSO (dimethyl sulfoxide) is used in PCR to facilitate the denaturation of DNA at high temperatures by destabilizing the secondary structure of DNA. It can also help to improve the specificity and yield of PCR reactions by preventing the formation of secondary structures that can inhibit the amplification process. Additionally, DMSO can help reduce the formation of primer dimers and nonspecific amplification products.
What do Australian Brumbies eat?
grass,pland and shrubs
