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When to Use dmso or glycerol?
DMSO (dimethyl sulfoxide) is commonly used as a cryoprotectant for freezing cells, while glycerol is often used as a stabilizer for enzymes and proteins. The choice between DMSO and glycerol depends on the specific application and the stability requirements of the biological material being used.
What is pcr and types of pcr?
PCR is a biotechnological method to amplify your gene (DNA) of your interest. It produce millions of your DNA fragments hence used in cloning. There are variants of this method using the same thermocycling principle such as touch down PCR, gradient PCR, RFLP, multiplex PCR, Q PCR, RT PCR and so on.
What are the possible impurities in dmso?
Possible impurities in DMSO (dimethyl sulfoxide) can include water, dimethyl sulfide, dimethyl sulfone, acetone, and other organic compounds. These impurities can affect the stability and purity of DMSO, altering its properties and potential uses. Regular purification methods such as distillation or filtration are typically used to remove impurities from DMSO.
What is the role of DMSO in cell culture?
DMSO (dimethyl sulfoxide) is commonly used in cell culture as a cryoprotectant to prevent ice crystal formation and cell damage during freezing and thawing of cells. It helps preserve cell viability and functionality when cells are stored at low temperatures. DMSO is also used as a solvent for some compounds and reagents in cell culture experiments.
Smell after using dmso?
DMSO can have a garlic-like smell when it reacts with compounds on your skin. This effect is temporary and should dissipate once the DMSO is fully absorbed or evaporated. It's important to use DMSO in a well-ventilated area to minimize the smell.
Why PCR need gelatin?
According to Roche website, different additives allow optimization to increase yield and specificity of PCR reactions. DMSO, for instance, is reported to reduce nonspecific priming, while gelatin and glycerol stabilize Taq DNA polymerase during PCR, which generally increases the yield.
Why is DMSO used as a control?
Many test compounds (drugs, inhibitors, etc) are not soluble in water and therefore dimethylsulfoxide (DMSO) is used as a solvent instead. The compound dissolved in DMSO is what is used to treat the cell or animal and therefore you must prove that it is the compound, not the DMSO which is causing any results seen. To ensure this, you have a control that contains only DMSO and not the test compound. This is often referred to as a "vehicle control".
When to Use dmso or glycerol?
DMSO (dimethyl sulfoxide) is commonly used as a cryoprotectant for freezing cells, while glycerol is often used as a stabilizer for enzymes and proteins. The choice between DMSO and glycerol depends on the specific application and the stability requirements of the biological material being used.
What are some common questions about PCR that researchers often encounter?
Some common questions that researchers often encounter about PCR include: How does PCR work? What are the different types of PCR techniques? What are the limitations of PCR? How can PCR results be validated? How can PCR be optimized for better results? What are the potential sources of error in PCR? How can PCR be used in different research applications? What are the ethical considerations when using PCR in research? How can PCR be used in clinical diagnostics? What are the current advancements in PCR technology?
What is the difference betwen DMSO and DMSO-d6 solution?
DMSO (dimethyl sulfoxide) is a common organic solvent, whereas DMSO-d6 is a deuterated form of DMSO used in NMR spectroscopy as a solvent. The "d6" indicates that the hydrogen atoms in DMSO have been replaced with deuterium, making it suitable for NMR analysis due to the absence of NMR-active protons.
What is pcr and types of pcr?
PCR is a biotechnological method to amplify your gene (DNA) of your interest. It produce millions of your DNA fragments hence used in cloning. There are variants of this method using the same thermocycling principle such as touch down PCR, gradient PCR, RFLP, multiplex PCR, Q PCR, RT PCR and so on.
Is DMSO a protic or aprotic solvent?
DMSO is an aprotic solvent.
Is DMSO a strong nucleophile?
Yes, DMSO is a strong nucleophile.
What are the possible impurities in dmso?
Possible impurities in DMSO (dimethyl sulfoxide) can include water, dimethyl sulfide, dimethyl sulfone, acetone, and other organic compounds. These impurities can affect the stability and purity of DMSO, altering its properties and potential uses. Regular purification methods such as distillation or filtration are typically used to remove impurities from DMSO.
What is the defference between Real-time PCR and reverse transcriptase PCR?
Difference between real time PCR and reverse transcription PCR is as follows:- 1. Real time PCR is donated as qPCR and on the other hand reverse transcription PCR is denoted as RT-PCR. 2. In qPCR, the template used is single strand DNA strand whereas in the RT-PCR, the template used in process is single strand of RNA. 3. The real time PCR enables both quantification as well as detection of the DNA in the real time whereas the RT-PCR enables only the quantification of the RNA and it is little bit slower process then the qPCR as it first produce the cDNA from the template RNA strand and then process it in the similar fashion as the traditional PCR.
What is the role of DMSO in cell culture?
DMSO (dimethyl sulfoxide) is commonly used in cell culture as a cryoprotectant to prevent ice crystal formation and cell damage during freezing and thawing of cells. It helps preserve cell viability and functionality when cells are stored at low temperatures. DMSO is also used as a solvent for some compounds and reagents in cell culture experiments.
What is the purpose of the mineral oil used for PCR when the termocycler does not have a hot lid?
To prevent evaporation of PCR products.
