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Bacteria are very tiny. Using a high powered microscope lens in necessary to view them properly, but at such high powers and close focusing, the 'picture' is often skewed by the air itself and it is easy to damage the lens. The oil provides a clearer focus by eliminating the air pocket between the lens and the specimen and also helps protect the lens from scratching and cracking.
What else can I help you with?
When you added a drop of iodine what effect did it have on the cell?
It stains the cell, making it easier to observe under microscope.
What color do bacterial endospores stain in the Schaeffer-Fulton spore stain?
Bacterial endospores stain green in the Schaeffer-Fulton spore stain due to the malachite green dye used. This technique is commonly used to visualize endospores in bacteria.
Why is it important to add iodine to cheek cells?
There is no complex chemical reason why iodine is added to a cell. It is simply added to stain a cell to make it easier to study under a microscope, as it gives some contrast to the cell to make certain organelles stand out that would be unclear or invisible without this stain. There are, however, many other stains that can be used, for example Haematoxyline and Eosin (H&E) staining which is used often to identify and study cancerous cells.
What does the gram stain in a cerebrospinal fluid analysis indicate?
The gram stain in a cerebrospinal fluid analysis indicates the presence of bacteria and helps to identify their morphology (shape and arrangement). This can provide valuable information for diagnosing bacterial meningitis or other bacterial infections in the central nervous system.
Is a stain on a microscope specimen used to turn it a pretty colour or not?
No, stains on microscope specimens are used to increase contrast and highlight specific structures or organelles. The color imparted by the stain helps differentiate different components of the specimen for easier observation under the microscope.
Is flagella viewable through an electron microscop or a light microscope?
Flagella are typically only visible through an electron microscope due to their small size, which is on the order of a few micrometers. Light microscopes do not have the resolution necessary to clearly visualize structures at this scale.
What is the pH of a carbolfuchsin direct stain?
The pH of a carbolfuchsin direct stain is typically acidic, around pH 2.5-3.0. This acidic pH helps the stain penetrate bacterial cell walls and bind to the cell structures, making them visible under a microscope.
What if You did not apply heat during the application of the primary stain?
Without heat, the primary stain may not penetrate the cell wall properly, leading to poor staining results. Heat helps to enhance the penetration of the stain into the bacterial cells, improving the visibility of the stain under the microscope. Thus, not applying heat during the application of the primary stain may result in weaker staining and difficulty in observing the bacterial cells.
What bacterial staining technique is it when a basic dye is utilized to stain bacterial cells?
The bacterial staining technique where a basic dye is used to stain bacterial cells is called simple staining. In this technique, the positively charged dye binds to the negatively charged bacterial cell structures, making them more visible under a microscope.
When you added a drop of iodine what effect did it have on the cell?
It stains the cell, making it easier to observe under microscope.
What is the function of a stain in microscope work?
As the cells are transparent, the components cannot be seen clearly through a microscope. We stain the cell so that we can see the components of the cell clearly through a microscope.
What is the purpose of covering the smear with bibulous paper when performing the endospore stain?
Covering the smear with bibulous paper during the endospore stain process helps to wick away excess stain and prevent the slide from drying out. This ensures that the endospores are properly stained and the background is clear for observation under the microscope.
What color do bacterial endospores stain in the Schaeffer-Fulton spore stain?
Bacterial endospores stain green in the Schaeffer-Fulton spore stain due to the malachite green dye used. This technique is commonly used to visualize endospores in bacteria.
What is it called to add dye to a specimen under a microscope?
to stain.
What is a stain that you can use to see things in a microscope easier?
iodine
Which diagram represent a cell organelle that can absorb iodine stain and then be seen with the low power of compound light microscope?
The diagram that represents a cell organelle that can absorb iodine stain and be seen with the low power of a compound light microscope is the nucleus. The nucleus absorbs the iodine stain and becomes visible under the microscope due to its high DNA content, which binds with the stain.
Can you heat fix blood smear before staining for bacterial examination?
You absolutely do not heat fix a blood smear before staining, that is, if you are looking at the blood cells. For bacteria, why wouldn't you culture it first and then heat fix, stain etc. I don't think heat fixing the blood stain would damage the bacterial cells so much as make it hard to differentiate the bacterial cells from the dead, shriveled, ruined blood cells, unless maybe you have like an electron microscope or something.
