as you zoom in the view gets bigger and if its not in the middle you wont be able to see it properly .
When a microscope is parcentered, the specimens will appear centered in the field of view at every magnification. So if a field of a slide is centered at the lowest power, even though the field diameter shrinks at each higher magnification, the desired part of the specimen will remain in the center of the viewing field.
A microscope has two lenses to magnify the image of the specimen. The objective lens, close to the specimen, captures a magnified image, which is further magnified by the eyepiece lens to enable viewing. This dual-lens system provides higher magnification and resolution.
Centering the specimen before switching to high power on a microscope helps ensure that the area you want to observe is in focus and at the center of the field of view. This is important because high power magnification has a narrower field of view and lower depth of field, making it more challenging to locate and focus on the specimen if it is not first centered.
The working distance decreases as magnification increases with a microscope. This is because higher magnifications require the objective lens to be closer to the specimen in order to achieve a clear focus.
Increasing the magnification of a microscope typically decreases the working distance, or the distance between the objective lens and the specimen. Higher magnification requires the objective lens to be closer to the specimen to achieve focus, reducing the working distance. Similarly, lower magnification allows for a greater working distance between the lens and the specimen.
When a microscope is parcentered, the specimens will appear centered in the field of view at every magnification. So if a field of a slide is centered at the lowest power, even though the field diameter shrinks at each higher magnification, the desired part of the specimen will remain in the center of the viewing field.
To increase the magnification of the specimen so you can see more detail.
A microscope has two lenses to magnify the image of the specimen. The objective lens, close to the specimen, captures a magnified image, which is further magnified by the eyepiece lens to enable viewing. This dual-lens system provides higher magnification and resolution.
Specimen magnification on a microscope is calculated by multiplying the magnification of the eyepiece by the magnification of the objective lens. For example, if the eyepiece magnifies 10 times and the objective lens magnifies 40 times, the total magnification would be 10 x 40 = 400 times.
by the objective lens
When changing magnification, the specimen may disappear from view due to the limited field of view at higher magnifications or improper focusing. If the specimen is not centered in the field before increasing magnification, it might fall outside the visible area. Additionally, if the microscope is not properly focused, the specimen may not be clear or may seem to vanish entirely. Adjusting the focus and centering the specimen can help resolve this issue.
To view a specimen under high power on a microscope, first ensure the specimen is properly mounted on a slide and in focus under low magnification. Then, increase the magnification to high power, typically 400x or higher, using the lens adjustment knob without contacting the slide. Finally, fine-tune the focus using the fine focus knob to get a clear view of the specimen at high magnification.
Centering the specimen before switching to high power on a microscope helps ensure that the area you want to observe is in focus and at the center of the field of view. This is important because high power magnification has a narrower field of view and lower depth of field, making it more challenging to locate and focus on the specimen if it is not first centered.
It means you have a good microscope.
The working distance decreases as magnification increases with a microscope. This is because higher magnifications require the objective lens to be closer to the specimen in order to achieve a clear focus.
Increasing the magnification of a microscope typically decreases the working distance, or the distance between the objective lens and the specimen. Higher magnification requires the objective lens to be closer to the specimen to achieve focus, reducing the working distance. Similarly, lower magnification allows for a greater working distance between the lens and the specimen.
Total magnification is the term used to describe the magnifying power of a microscope, which is calculated by multiplying the magnification of the objective lens by the magnification of the eyepiece. This formula helps in determining the overall magnification of the specimen being viewed under the microscope.