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  • Dissolved oxygen is measured by the Winkler titration method.
  • BOD5 is measured by putting a known volume of bacteria in a sample of the fluid to be insppected at the saturation point of oxygen, keeping it at a specified temperature for 5 fays then measuring the oxygen content.
Both procedures are found in the "Standard Methods for the Examination of Water and Wastewater"
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In the BOD (Biochemical Oxygen Demand) test, a blank is used to account for any oxygen demand exerted by materials other than the sample itself, such as the reagents and the dilution water. This ensures that the measured BOD reflects only the oxygen consumed by the biodegradable organic matter present in the sample. By subtracting the BOD of the blank from the sample's BOD, the results provide a more accurate assessment of the sample's organic pollution load.


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