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Yes, PBS (phosphate-buffered saline) can be used as a mobile phase in a C18 column, particularly for reverse phase chromatography. However, it is important to consider the pH and ionic strength of the PBS, as these factors can influence retention time and separation efficiency. Additionally, the presence of salts in PBS may affect the column’s performance and longevity, so it is advisable to validate the method for specific applications.

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3w ago

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