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Yes, Mary Queen of Scots and Mary I of England were related. Mary Tudor's father was Henry VIII - and his sister, Margaret Tudor, was Mary Queen of Scots' grandmother (she married James IV of Scotland). This family connection was the basis for Mary Queen of Scot's claim to the English throne which would shape Anglo-Scottish relations during the 1540s-1560s, and the reason for the strained relationship between Mary Queen of Scots and Elizabeth.

So, the answer below does not take into account the fact that the Tudor and the Stewarts were related on Mary Queen of Scots' father's side!!

No. However Queen Mary I ( Bloody Mary) who only reigned 5 years-l553-58 and was succeeded on her death by Queen Elizabeth I- were half-sisters. they had the same father- Henry The Viii! Mary was the daughter of Katharine of Aragon who was a Spanish Princess, ( never called by her Spanish handle, Catalina) and Elizabeth of the ill-fated Anne Boleyn. Both had Henry as father. Mary Stuart, the Queen of Scots ws the daughter of the ruling Queen of France, Marie de Guise, and was not a blood relative of the English Royals at all.

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