Yes.
The questions is not quite as simple as it sounds, though.
The Mason-Dixon Line was the agreed-upon boundary between Maryland and Pennsylvania, and between Maryland and Delaware. It did not mark the boundary between slave and free states when it was drawn in the 1760s -- in fact slavery was legal in all of the colonies at that time.
Later, in 1820 (at the time of the Missouri Compromise), the term 'Mason-Dixon Line' came to indicate the cultural and political divide between north and south. Delaware was a slaveholding state at that time, but the slave-keepers were not in control of the political dealings of the state as they were in nearly all other slave states.
The Mason-Dixon Line remained a symbol name for the dividing line between free and slave states, and later between north and south when slavery was ended. Most people today (outside of history class) have no idea where the line actually stood or what it really meant.
Some of the carved stone markers that Mason and Dixon placed from 1763 to 1767 are still in their original places, with the seals of the Penn family, Maryland, and Delaware still visible. See the related links for an example.
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Its South of the Mason-Dixon line, a very small part is north of it. (even parts of New Jersey, are South of the line.)
Maryland Kentucky Delaware Missouri
Kentucky, Maryland, Missouri, Delaware
Delaware Kentucky Maryland Missouri and west Virginia
The Mason-Dixon Line.
New York was the eleventh of the original thirteen colonies to become a state. New York officially became a state on July 26th, 1788.