5' end (nucleotides are added from 3' toward 5')
During DNA synthesis, nucleotides are added in a specific directionality, moving from the 5' to the 3' end. This means that new nucleotides are added to the growing DNA strand in a continuous manner, with the 5' end of the new nucleotide attaching to the 3' end of the existing strand.
During DNA replication, nucleotides are added to the 3' end of a DNA strand by an enzyme called DNA polymerase. This enzyme attaches new nucleotides to the existing strand in a complementary manner, following the base pairing rules (A with T, and G with C). The 3' end of the DNA strand provides a free hydroxyl group (-OH) that allows the DNA polymerase to add the new nucleotide, extending the DNA strand in the 5' to 3' direction.
During DNA synthesis, new nucleotides are added to the growing DNA strand in the 5' to 3' direction. This means that nucleotides are added to the 3' end of the existing strand, as DNA polymerase can only add nucleotides in this direction. This process ensures that the new DNA strand is synthesized in the correct orientation and maintains the genetic information encoded in the original DNA template.
Nucleotides are added during DNA replication to create a new complementary strand of DNA, ensuring accurate genetic information is passed on to daughter cells during cell division.
During DNA replication, nucleotides are numbered based on their position in the DNA strand. The process involves the separation of the DNA double helix into two strands, with each strand serving as a template for the synthesis of a new complementary strand. As new nucleotides are added to the growing strand, they are numbered sequentially to ensure the accurate replication of the genetic information. This numbering helps maintain the integrity and fidelity of the DNA replication process.
The DNA strand that acts as a pattern for the newly synthesized DNA is called the template strand. It serves as a guide during DNA replication, where complementary nucleotides are added to create a new DNA strand.
Incorrect nucleotides added during DNA replication can be repaired by enzymes that detect and remove the mismatched nucleotides, a process known as mismatch repair. This mechanism helps maintain the accuracy of the DNA sequence.
DNA is synthesized in the 5' to 3' direction. This means that nucleotides are added to the 3' end of the growing DNA strand. DNA polymerases, the enzymes responsible for DNA synthesis, can only add nucleotides to the 3' hydroxyl group of the existing strand. As a result, the template strand is read in the 3' to 5' direction during replication.
The continually elongating strand of new DNA at one side of a replication fork during DNA replication is called the leading strand. It is synthesized continuously in the 5' to 3' direction by DNA polymerase.
NEED OF PRIMER IN PCR-It is because the polymerase enzyme we use in the PCR only extend a DNA strand but not initiate its synthesis. So, to initiate the synthesis of DNA strand onto a template strand we require primers.
The sequence of nucleotides in the template DNA strand determines which complementary nucleotide will be added to the growing strand. A-T and G-C base pairing rules govern the selection of the nucleotide to be added during DNA replication.