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by heating above certain temprature eg.90 or 100 degree celcius or by treting with strong alkali or strong acid you can denature your DNA
*Actually, you can denature DNA in water if you wanted to. Basically any polar solvent will denature DNA because it has a negatively charged sugar-phosphate backbone. Mutagens can also influence DNA although it isn't exactly denaturing it. So can high energy light, like UV or all kinds of radiation. This, too, isn't denaturing though.
Wiki User
∙ 13y ago
Wiki User
∙ 14y agoby heating upto 92*-95*
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Why must DNA fragments be exposed to an alkaline solution before hybridization?
To denature the DNA
What is the first step in the polymerase chain reaction?
It allows the biologists to make many copies of a dna strand...
What are four factors or substances that can denature proteins?
Temperature (high), Research Gel electrophresis (method for breaking up DNA)
What is the role of urea in DNA extraction?
Urea is a chaotropic agent, and its role is obviously denature proteins and DNA, and promote more stability to the system, breaking the hydrogen ligations between DNA and water and making the intramolecular ones more stronger.
What does ctab use for isolation of DNA from animal blood?
CTAB is a detergent used to denature proteins from samples. Once the protens have been denatures, the isolation of DNA from the non required waste materials can begin.
What is the function of formamide loading buffer?
To denature DNA
Why must DNA fragments be exposed to an alkaline solution before hybridization?
To denature the DNA
How does formamide denature DNA?
Through the magic of hydrogen bonding
Why is cold ethanol better than room temperaure for DNA isolation?
DNA does not dissolve on Alcohols. It is better to store it in cold temp. for it not to denature
What is the role chelex-100 in DNA extraction?
Chelex is there to simply denature the Double stranded DNA and convert it to single strand DNA so the primers can attatch to it during PCR.
Does boiling denature cells?
Boiling can allow for the extraction of DNA.
What is the first step in the polymerase chain reaction?
It allows the biologists to make many copies of a dna strand...
Can you tamper with DNA?
Not directly. Radiation can cause mutations in DNA. Excess heat (as in the case of a fever) can denature (destroy) the DNA sequence as well as other proteins which will usually result in cell death.
How does hot water work to release DNA inside the cell nuclei?
The cells are incubated in a hot water bath in order to denature the cytoplasmic enzymes which break apart DNA.
What are four factors or substances that can denature proteins?
Temperature (high), Research Gel electrophresis (method for breaking up DNA)
What is the role of Triton in DNA isolation?
it is non-ionic detergent.so it act as non-denaturing agent and membrane protein are not denature.
What is the role of urea in DNA extraction?
Urea is a chaotropic agent, and its role is obviously denature proteins and DNA, and promote more stability to the system, breaking the hydrogen ligations between DNA and water and making the intramolecular ones more stronger.
