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At concentrations above 1% phenol has a significant antibacterial affect. Derivatives of phenol, called phenolics, injure the lipid-containing plasma membranes of bacteria and cause the cell to leak its cellular contents.
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Phenol coefficient test in microbiology?
Phenol coefficient test is best known screening test in which potency of a disinfactant is compared with that of phenol. A series of a dilutions of phenol and the disinfactant being tested are prepared. A standard amount of Salmonella tyhpii and Staphylococcus aureus are added to each dilution; the dilutions are then placed in a 20 to 37oC water bath. At 5-minute intervas, samples are withdrawn from each dilution and used to inoculate a growth medium, which is incubated for two or more days and then examined the growth. If there is no growth in the growth medium, the dilution at that particular time of sampling killed the bacteria. The highest dilution that kills the bacteria after 1o min. expousre, but not at 5min. is used to calculate the phenol coefficient. This is done by dividing the resiprocal of the appropriate phenol dilution.
How does good bacteria in marmite work?
bacteria in marmite
What is the bodys first defense against bacteria and viruses?
Lysozymes
What is the substrate for phenolase?
The substrate for the enzyme phenolase is phenol.
Are non specific defence mechanisms more effective than specific defense mechanisms if so why?
Non specific defense mechanisms are more effective because they work against all bacteria and viruses. Examples are skin or tears. Specific defense mechanisms like antibodies work against one type of bacteria or virus. They're needed if a pathogen gets past the nonspecific defense mechanisms.
Do antibiotics work against bacteria?
antibiotics are useful against bacteria because they help to kill off the nasty bacteria or they can also stop the bacteria from reproducing - so the illness doesn't get worse. this then gives your body time to make antibodies which will eventually distroy the bacteria. after this, you won't get the disease again because you are immune to it.
Why does an antibiotic not work the same for all bacteria?
Bacteria also do evolve. If one bacteria is mutated, and survives an attack by antibiotic, he multiplies and forms more bacteria which are more resistant against antibiotic. As days of surviving antibiotics and multiplying eventually creates a bacteria which is resistant against it.
What is the use of chick-Martin test?
Chick martin test is used to estimate the efficacy of a disinfectant. In this test a standard culture of Salmonella typhi, is tested for a fixed period (30 minutes) against various concentrations of phenol solution and various concentrations of the testing disinfectant; the result is expressed as the phenol coefficient, the highest dilution of the given disinfectante that kills bacteria, divided by the highest dilution of phenol that sterilizes the solution, within the measured time.
What is the use of chick martin test?
Chick martin test is used to estimate the efficacy of a disinfectant. In this test a standard culture of Salmonella typhi, is tested for a fixed period (30 minutes) against various concentrations of phenol solution and various concentrations of the testing disinfectant; the result is expressed as the phenol coefficient, the highest dilution of the given disinfectante that kills bacteria, divided by the highest dilution of phenol that sterilizes the solution, within the measured time.
When are antibiotics not effective?
An antibiotic might not work because the symptoms are attributed to a virus rather than a bacterium. Antibiotics do not work against viruses.
What is the role of beta-mercaptoethanol in DNA extraction?
stabilization of phenol against oxidation
Why are antibiotics not given unless a serious infection?
Bacteria can become immune to antibiotics and the antibiotics will not work in the future when you need them. They only work against bacteria and cold and flu are caused by viruses.
What type of antoboitics work against viruses?
It's called NONE. Antibiotics are for bacteria. They are not for virus because they don't work on virus.
Phenol coefficient test in microbiology?
Phenol coefficient test is best known screening test in which potency of a disinfactant is compared with that of phenol. A series of a dilutions of phenol and the disinfactant being tested are prepared. A standard amount of Salmonella tyhpii and Staphylococcus aureus are added to each dilution; the dilutions are then placed in a 20 to 37oC water bath. At 5-minute intervas, samples are withdrawn from each dilution and used to inoculate a growth medium, which is incubated for two or more days and then examined the growth. If there is no growth in the growth medium, the dilution at that particular time of sampling killed the bacteria. The highest dilution that kills the bacteria after 1o min. expousre, but not at 5min. is used to calculate the phenol coefficient. This is done by dividing the resiprocal of the appropriate phenol dilution.
Structure of phenol?
phenol
Is phenol volatile?
Phenol is flammable.
Which is stronger acid ethanol or phenol?
phenol
