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How long should you wait before using the floor after applying water base polyurethane?

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This will depend on the humidity and temperature of the room, but generally 24 hours.
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Can I use Water based polyurethane over flat wall paint?

No. Poly and paint are incompatible. Instead use liquid floor wax. It's easier to work with and will give you a longer lasting surface that doesn't stick to paPer when wet. Yo

Can you put polyurethane on Water based paint?

How old is the water based paint? If it has been dried for a few years, you will probably be OK, but I would avoid it if you can.  You can also find a water based polyurethan

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