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If iodine step was amitted how would gram negative cells appear?
Wiki User
∙ 14y ago
Iodine work as modrant to crystal violet if the iodine step will be emitted then the crystal violet will not be able to show its effect and all cells will appear pink when counter stain with saffranin
Wiki User
∙ 14y ago
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Why use iodine when preparing slide for viewing?
one disadvantage of using iodine to stain cells is that iodine will kill living cells.
What does effect iodine have on the onion cells?
iodine stains the onion cells so that you can see the cell better. you can see the nuclei and cytoplasm.
Do onion cells still have chloroplasts when they have iodine on them?
The iodine has no connection as to whether the cells have chloroplasts or not. If the cells are green, then they do have them. Adding iodine doesn't change that fact. Not all cells of the plant have chloroplasts. The ones in the soil don't so if you are working with the bulb, it doesn't have chloroplasts in the first place.
What are effects of staining onion cells with dilute iodine solution?
It affects the end results. If you look at the cell under a microscope, you will see that the section where the solution of the iodine has been placed it will appear different from the others. It might be a seperate colour or it could vary in size.
What is the administration of radioactive iodine to destroy thyroid cells?
chemical thyroidectomy
Why is methylene blue is an appropriate dye for staining bacterial cells?
It is used in gram staining to differentiate gram negative and gram positive bacteria. After being dyed, the cells are washed with ethanol. Gram positive bacteria will retain the methylene blue due to the amount of peptidoglycan in their cell walls, where gram negative cells will not. Iodine is used as a counter stain, which is up-taken by gram negative cells. After the gram staining procedure is finished, gram positive cells will appear dark purple or blue due to the retained methylene blue. Gram negative cells will appear pink or red due to the iodine counter stain.
When iodine solution is dropped onto plant cells why do dark blue spots appear?
Dark blue spots appear to show that there is starch on the plant cells. -Anonymous-
Why do you use iodine to stain a cell?
Iodine stains starch contained in cells. Iodine is also used to distinguish between Gram negative and Gram positive bacteria. The Gram stain contains iodine.
Why use iodine when preparing slide for viewing?
one disadvantage of using iodine to stain cells is that iodine will kill living cells.
What does effect iodine have on the onion cells?
iodine stains the onion cells so that you can see the cell better. you can see the nuclei and cytoplasm.
Do onion cells still have chloroplasts when they have iodine on them?
The iodine has no connection as to whether the cells have chloroplasts or not. If the cells are green, then they do have them. Adding iodine doesn't change that fact. Not all cells of the plant have chloroplasts. The ones in the soil don't so if you are working with the bulb, it doesn't have chloroplasts in the first place.
What is the radioactive iodine called used to destroy thyroid cells?
The thyroid processes iodine.
What are effects of staining onion cells with dilute iodine solution?
It affects the end results. If you look at the cell under a microscope, you will see that the section where the solution of the iodine has been placed it will appear different from the others. It might be a seperate colour or it could vary in size.
Why is why it is helpful to use dyes such as iodine to view cells in a microscope?
They are usd b'cos the dyes have positive electrons which react with the negative in the specimens therby giving them colour or no colour 2 be observed & identified.
If your organisms were all blue with pink spots what is the Gram reaction if your organisms were all pink but had some blue spots what is the Gram reaction?
Gram positive cells take up the crystal violet, which is then fixed in the cell with the iodine mordant. This forms a crystal-violet iodine complex which remains in the cell even after decolorizing. It is thought that this happens because the cell walls of gram positive organisms include a thick layer of protein-sugar complexes called peptidoglycans. This layer makes up 60-90% of the gram positive cell wall. Decolorizing the cell causes this thick cell wall to dehydrate and shrink, which closes the pores in the cell wall and prevents the stain from exiting the cell. At the end of the gram staining procedure, gram positive cells will be stained a purplish-blue color. Gram negative cells also take up crystal violet, and the iodine forms a crystal violet-iodine complex in the cells as it did in the gram positive cells. However, the cell walls of gram negative organisms do not retain this complex when decolorized. Peptidoglycans are present in the cell walls of gram negative organisms, but they only comprise 10-20% of the cell wall. Gram negative cells also have an outer layer which gram positive organisms do not have; this layer is made up of lipids, polysaccharides, and proteins. Exposing gram negative cells to the decolorizer dissolves the lipids in the cell walls, which allows the crystal violet-iodine complex to leach out of the cells. This allows the cells to subsequently be stained with safranin. At the end of the gram staining procedure, gram negative cells will be stained a reddish-pink color. Remember:
A what treatment is the oral administration of radioactive iodine to destroy thyroid cells?
Addison's disease is treated using the oral administration of radioactive iodine to destroy thyroid cells.
What is the purpose of mordant in a differential staining procedure?
The resultant crystal violet- iodine (CV-1) complex serves to intensify the-color of the stain. At this point, all cells will appear purple black.
