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PCR stands for Polymerase Chain Reaction. It is a process used to take a small piece of DNA and use it to reproduce copies of a particular genomic sequence. This is useful in testing for genetic diseases, forensic science, and paternity testing.
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What organism is used primarily in PCR technique?
well in PCR technique it is a part of DNA whcih is used to polymerized or multiplied but for studing Genetic engineering we generally prefer most studied microorganism i.e. E.coli
What is the use of maintaining a certain pH in pcr?
PCR is an enzymatically guided process. In optimum pH the enzyme will work best.
What is pcr and types of pcr?
PCR is a biotechnological method to amplify your gene (DNA) of your interest. It produce millions of your DNA fragments hence used in cloning. There are variants of this method using the same thermocycling principle such as touch down PCR, gradient PCR, RFLP, multiplex PCR, Q PCR, RT PCR and so on.
How has the lab technique PCR helped scientists study evolutionary relationships?
PCR helps, specifically, with finding homology between sequences of dna quickly and spefically where scientists want to look in the genomes. PCR, by using 'primers,' pinpoint exactly where they want to make copies of DNA. By making these copies, they can be read by a machine and from there, 'multiple sequence alignment' can be performed to examine evolutionary relationships. These sequences and homology matter to scientists such as structural biologists who want to find out the specific 3D shape of homologous proteins or RNA. They can use homology in, say mice (mammal) DNA sequences to make predictions about the shape of human proteins/RNA--therefore finding ways to attack a 3D structure with a new synthetic medicine.
PCR stands for?
PCR stands for Polymerase Chain Reaction.
What is the use of dNTP?
The use of dNTP is PCR and multiplex PCR
What is a Real Time PCR and what does it offer?
It is a very helpful tool. It is usually used by scientists. They use it to determine something or make certain studies. Real Time PCR offers various effective tools that could be very helpful.
Why do you use a negative control in PCR?
to check is there any contamination in pcr products
Why are scientists so interested in extremophiles?
Extreme environments have been useful to scientists in inventing PCR. It was in an extreme environment like the geysers of Yellowstone that a scientist discovered that a bacteria was living in the extremely hot water and yet still could function. Before PCR we knew we could separate a strand of DNA by heating it, but there was no polymerase to duplicate it that would work at such a high temperature. The bacteria in the hot water had a polymerase that would. So now scientists use that to do PCR and create many copies of DNA.
Use of water in pcr?
enzyme cofactor
What are the different types of polymerase chain reaction techniques?
types of pcr: AFLP -PCR. Allele-specific PCR. Alu-PCR. Assembly -PCR. Assemetric -PCR. Colony -PCR. Helicase dependent amplification. Hot start pCR. Inverse -PCR. Insitu -pCR. ISSR-PCR. RT-PCR(REVERSE TARNSCRIPTASE). REAL TIME -PCR
What organism is used primarily in PCR technique?
well in PCR technique it is a part of DNA whcih is used to polymerized or multiplied but for studing Genetic engineering we generally prefer most studied microorganism i.e. E.coli
What is a Taqman Real Time PCR?
One can use a PCR to amplify and quantify a certain DNA molecule. A TaqMan real-time PCR is a certain type of PCR which uses the TaqMan method to increase its specificity.
What is the use of maintaining a certain pH in pcr?
PCR is an enzymatically guided process. In optimum pH the enzyme will work best.
What do physician use to screen a genetic disorder?
PCR
What equipment is use for collection and detection of biological agents?
PCR
What is pcr and types of pcr?
PCR is a biotechnological method to amplify your gene (DNA) of your interest. It produce millions of your DNA fragments hence used in cloning. There are variants of this method using the same thermocycling principle such as touch down PCR, gradient PCR, RFLP, multiplex PCR, Q PCR, RT PCR and so on.
