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See related link below for a better explanation than mine..

I am not to familiar with Rf values, but I have an idea. Rf values are the ratio between the distance traveled by the spot of the analyte to the distance traveled by the solvent used. This is used in paper chromatography and thin layer chromatography. Rf value will allow you to compare to your unknown substance that you are trying to analyze.

I will put a link the both paper and TL chromatography.

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13y ago
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11y ago

· Used in identification pusposes

· Use to determine the affinity of the solute to the solvent

- Greater Rf , greater affinity of solute to the solvent

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Q: What is the significance of Rf value in chromatography?
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What is an rf value used for?

The rf value is shorthand for the retention value of a substance. It is used in chromatography to determine the components of an unknown sample.


How do you work out RF value?

Rf = distance to color/distance to front line


How do you measure Rf?

Rf value = distance traveled by a compound/distance traveled by a constant.Source: http://www.chemguide.co.uk/analysis/chromatography/paper.html


What is Rf value?

It is the distance travelled by the sample or analyte divided by distance travelled by the solvent front in chromatography.


How do you calculate Rf in chromatography?

In chromatography, Rf is the distance the solute travels divided by the distance the mobile phase travels. For example, in thin layer chromatography, if the spot travels 7 cm, and the mobile phase travels 15 cm, the Rf value for that spot will be 7/15 = 0.47


Define the Rf value?

RF value (in chromatography) The distance travelled by a given component divided by the distance travelled by the solvent front


How is rf factor in chromatography calculated?

It's not.


What will happen if there is an overrun of solvent in paper chromatography?

The Rf value cannot be determined since the distance of the solvent may overlay in the paper.


Would RF values of amino acid you use in paper chromatography be the same if you use another solvent system?

Rf value is the ratio of distanced covered by solute by distanced covered by solvent.


A compound has a very high Rf value in a polar solvent what can you say about its molecular structure?

Rf Value - It tells you the affinity that the compound has to the solvent...High Rf = little or no polar groups and low Rf = more polar groups. Question: In TLC (thin-layer chromatography) when the compound has a high Rf the solvent is: a. less polar b. Is more polar Answer: a. less polar


What is the Significance of Rf value?

· Used in identification pusposes · Use to determine the affinity of the solute to the solvent - Greater Rf , greater affinity of solute to the solvent


What do the Rf values indicate about the relative solubility of the pigment in the the solvent?

The Rf values do not indicate the solubility of a substance. The Rf value or retardation factor is the ratio of the distance traveled by the center of a pot to the distance traveled by the solvent front in chromatography.