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A plasmid is a piece of circular DNA. These plasmids are transformed into bacteria/yeast.
A library is made by taking pieces of DNA (from a genomic DNA digest, or cDNA) and inserting them into plasmids. The plasmids are then transformed into the organism and stored. Libraries are used to screen for new functional genes, usually when looking at new substrates or products that the yeast/bacteria can't make without the plasmid.
Wiki User
∙ 13y ago
Wiki User
∙ 13y agoA plamid library is a collection of DNA fragments cloned into a plasmid vector (a typically circular DNA element that can be replicated in a bacterial host and carry an inserted sequence). Escherichia coli bacteria are transformed with the collection of plasmids and single colonies (each containing a different insert) are grown and stored frozen separately. This allows isolation of a plasmid with a specific sequence for further analysis. The library can be used e.g. for screening, i.e. looking for plasmids that contain inserts with a specific property that can be detected by the screen.
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When is a plasmid considered a recombinant plasmid?
When the original function of the gene in the plasmid is altered or another gene is inserted in the non- coding region of the plasmid is called the recombinant plasmid.
Analogy for plasmid?
F-plasmid (fertility plasmid) of any conjugative bateria or Ti-plasmid (tumor-inducing) of Agrobacterium tumaefaciens.
What occurs first in the production of a recombinant plasmid?
Isolation of a plasmid from a bacterium
What type of plasmid is the source of the problem with antibiotic resistance?
R plasmid.
What is the bacterial cell called when the f factor is in plasmid form?
F Plasmid
When is a plasmid considered a recombinant plasmid?
When the original function of the gene in the plasmid is altered or another gene is inserted in the non- coding region of the plasmid is called the recombinant plasmid.
What are some of the characteristics of cosmid?
A cosmid has the characteristics of a hybrid plasmid. They are used in the building of genomic libraries. They are ~200 base pairs long. More detailed information is available on Wikipedia.
What is the function of the Ti plasmid?
what is the function of the plasmid
Which is the plasmid that increases resistance to antibiotics?
R-plasmid
What plasmid that can degrade toluic acid?
TOL plasmid
What are BACs and YACs?
BAC = Bacterial Artificial Chromosome -200-300Kb -good for genomic libraries -uses the bacterial F (sex) plasmid -currently quite popular YAC = Yeast Artificial Chromosome -1Mb -good for genomic libraries also -difficult to manipulate and use -less popular
Analogy for plasmid?
F-plasmid (fertility plasmid) of any conjugative bateria or Ti-plasmid (tumor-inducing) of Agrobacterium tumaefaciens.
What is a self transmissible plasmid?
A plasmid which encodes genes for its own transfer.
What is a self-transmissible plasmid?
A plasmid which encodes genes for its own transfer.
A plasmid is a type of?
Plasmid is extrachromosomal DNA capable of self replication.
What type of plasmid is the source of the problem with antibiotic resistance?
R plasmid.
What occurs first in the production of a recombinant plasmid?
Isolation of a plasmid from a bacterium
