What are the benefits of extracted DNA from banana?
the benefit to extract DNA from banana IS TO PRODUCE NEW AND MORE RAPID POPULATION OF FRUITS
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There are three basic steps in a DNA extraction, the details of which may vary depending on the type of sample and any substances that may interfere with the extraction and subsequent analysis.. Chelate divalent cations such as Mg 2+ and Ca 2+ to stop dnase enzymes functioning and degrading the D…NA . Break open cells by grinding or sonication, and remove membrane lipids by adding a detergent. . Precipitate DNA in cold ethanol or isopropanal, DNA is insoluble in alcohol and clings together; this step also removes salt. (MORE)
Mutating a particular DNA sequence into something more interesting. Probing the DNA for known sequences. Digesting the DNA into the little pieces in order to analyze them.
DNA can be extracted from strawberries. DNA can be extracted fromvarious things not just food because it is found in all livingthings.
you have to get a lobster to bite the tree then suck the bark off then squeeze a banana over it
The most common uses of extracted DNA include: - amplifying a small amout of DNA (or small sequence) into an enormous amount - mutating a particular DNA sequence into something more interesting - probing the DNA for known sequences - digesting the DNA into little pieces in order to analyze t…hem Extracting DNA from cells is one of the first steps of one of the most commonly used procedures in molecular biology: Polymerase Chain Reaction (PCR). Separating the DNA from the rest of contents of the cell makes for a cleaner result, but nowadays it's not strictly required. Extraction of DNA is important because of many reasons. With the ability to remove DNA from an organism, scientists can observe, manipulate, and classify the DNA. Scientists can identify genetic disorders or diseases from studying DNA. Scientists can possibly find cures for these causes by manipulating or experimenting with this DNA. Scientists can accurately sort organisms into classes because of DNA uniqueness. If we didn't have DNA extraction, it would be a lot harder to decide which organisms are different from each other. Scientists can genetically engineer some organisms to produce beneficial things. A common example is that of insulin. Scientists can genetically engineer insulin production so that people with diabetes can live longer. (MORE)
To extract DNA from a person you need it in liquid form (i.e. spit) or if it's from a plant you can just grind up the plant in a blender to open up the cells. You need to add detergent and meat tenderizer. Detergent breaks down the lipid bilayer and meat tenderizer breaks down the protein surroundin…g the DNA. Let it sit for about 15 minutes so that everything can be broken down. Then add some isopropyl alcohol to it. The alcohol is polarized. It has a positive charge and DNA is negative by nature so it will pull the negative DNA out of everything else. You will see little bubbles or strings of white stuff forming and in a moment you will have clumps of DNA. This is as pure as you can get without being in a lab because they have stronger detergents and enzymes. (MORE)
The only DNA in blood would be the DNA contained in white blood cells as red blood cells have no nucleus and therefore no DNA.. Extracting DNA from blood is very easy in a laboratory nowadays. The sample of blood is treated with detergants to break open the cell membrane spilling the contents. Enzy…mes are now used to break down all the protein, RNA, sugars and fats in the solution. Ethanol (alcohol) is often used in the final stages of DNA extraction as under the right conditions as DNA will dissolve into it but other componants of the cell will not allowing the separation of DNA to be used for analysis (MORE)
SDS is strong anionic detergent. It removes the -ve ions from theprotein and destroys its confirmation. Because of loss ofconfirmation the protein loses its structure. The proteins fro thecel membrane get damaged and cell gets broken. SDS which stands for 'sodium dodecyl sulfate' is a strong anionic…detergent that can solubilize the proteins and lipids that form themembranes. This will help the cell membranes and nuclear envelopesto break down and expose the chromosomes that contain the DNA.â¨â¨â¨â¨In addition to removing the membrane barriers, SDS helps releasethe DNA from histones and other DNA binding proteins by denaturingthem. (MORE)
For many many different reasons.. 1)The tissue sample may be examined to determine the genetic code of a specific gene(s) being studied and to compare the sequences for a large number of samples, for example cancer biopsys and non-cancerous tissues.. 2)The tissue maybe examined by DNA sequencing t…o detect SNP's causative of different diseases or showing spatial/racial groupings. (MORE)
I can't tell you how it is done in a high-tech lab, but this low-tech way will work! 1. Since we are dealing with a plant cell (such as peas), the cell walls need to be broken. This is done by adding water to the peas (or whatever you are using), and blending them for a few seconds. 2. Add a small… amount of Dawn dishwashing liquid (breaks the cell and nuclear membranes). 3. Add a pinch of meat tenderizer (separates the proteins from the DNA). 4. Stir gently. 5. Put about an inch of the material into a test tube. 6. Carefully add about the same amount of rubbing alcohol so that the alcohol forms a layer on top of the "pea soup." 7. Since DNA is less dense than the "pea soup," it will rise and it enters the alcohol layer and appears as a white stringy material. Try googling "how to extract DNA from anything living." DL (MORE)
DNA extraction is done by three methods:. Organic extraction . inorganic extraction . solid state method . In organic extraction, phenol and chloroform are used to create on organic phase in which cells are lysed and DNA is freed. The DNA remains in the aqueous phase. Ethyl alcohol is used to p…recipitate the DNA.. In the inroganic methos, NaCl and EDTA are used for cell lysis. Following this, an approach similar to the organic method is followed.. In solid state extraction, DNA is first precipitated in the presence of high slat and low pH conditions. The precipitated DNA is then adsorbed on to a filter membrane surface. (MORE)
Meat tenderizer does not in itself extract DNA. Tenderizer is an enzyme ( usually a bromeliad) which helps break down the proteins in the cell.
RNAse destroys the RNA and hence RNAse contamination is a problem in RNA extraction as it breaks down RNA. RNAse enzyme is removed by using RNAse inhibitor or precautions like wearing of gloves, autoclaving tips , using RNAse free water/DEPC treated water is done while performing RTPCR
Lysozyme is an enzyme that is used during DNA extraction to degrade the cell wall.
You mash the fruit so you can break through the cell wall and the DNA can be extracted.
The salt neutralizes the DNA's negative charge with its positive charge while the DNA precipitates.
salt mainly NaCl is used for the separation of cTAB molecules from DNA precipitated out by the help of cTAB
TE stands for Tris and EDTA. The Tris buffers the water to prevent acid hydrolysis of the DNA/RNA. The EDTA chelates divalent cations that can assist in the degradation of RNA.
Saliva is a watery secretion of the mouth...so it does not contain DNA but ..the saliva contains cells..like cheek epithelial cells from which DNA can be extracted.
DNA is a non-living molecule to begin with. It has no life to it. It is merely a chain of molecules called nucleotides. If isolated correctly and frozen, DNA will retain its integrity (be in an unbroken state) for a few months. If stored in liquid nitrogen, DNA can be stores for several years …after isolation. (MORE)
you get a banana with the peel still on you cut it in half the long way then you get a pot and put 4 cups of water in it then get it boiling boil the banana for 15-20 minutes then you take the banana out and the liquid left in is your banana extract
Ear wax contains very few cells. It is mainly complex carbohydrates and other waste materials. The lack of cells makes it nearly impossible to isolate DNA from ear wax
For humans, blood is easily accessible. There are standard procedures to safely obtain blood from an individual and DNA can be extracted from the white blood cells. However, DNA can also be obtained from hair and salivary swabs but these are not considered tissues.
Very little fat and sodium A good amount of potassium, magnesium, vitamins C and B-6, and fiber.
When DNA is an an aqueous solution, is is surrounded by water molecules. These surrounding molecules constitute the shell of hydration. By adding salt, the shell of hydration is replaced by salt molecules and the DNA comes out of solution. The DNA is now said to be in a precipitated state. The proce…ss is commonly referred to as 'salting out' (MORE)
It breaks down the Cell Membrane, making it easier for the DNA to come through. Good Luck :)
This is the concept of something that would be used in a laboratory setting: Collect a lot of cells that you would want to extract DNA from. Break the cells using a detergent-soap or something basic, or something physically destructive like a blender. Neutralize the reaction with vinegar or… some acid. Collect the clarified supernatent, and precipitate it with isopropyl alcohol. Remove the IPA and add ethanol to wash Resuspend again in a buffer or water. Now, if you want to do it at home... Collect cells or any material that has DNA Blend them to break them into smaller pieces Add soap detergent to break the cells apart. Add contact lens solution. Add rubbing alcohol, as high percentage as possible. And that's it! (MORE)
Alcohol is added to the aqueous solution of DNA at the end point of the extraction process, it precipitates out the DNA as DNA is insoluble in alcohol.
What you would want to do is dry the banana peel out much like a dried banana chip then you will want to use a press and put the banana peel under highpressuer I use a 3 ton jack press thats in my basement for this method ...how ever dried banana chips are much easyer to do than the actual peel
Mutanolysin provides gentle cell lysis for the isolation of easily degradable biomolecules and RNA from bacteria. It has been used in the formation of spheroplasts for isolation of DNA. It is a muralytic enzyme that cleaves the N-acetylmuramyl-Î²(1-4)-N-acetylglucosamine linkage of the bacterial c…ell wall polymer peptidoglycan-polysaccharide. (MORE)
When extracting DNA from a banana, the DNA must release from the cell by breaking apart or lysing the cellular and nuclear membranes. Lysing in this case is the act of breaking open the cell membranes to expose the contents. This is performed by mashing the banana and adding a detergent/salt solutio…n. (MORE)
It provides Osmotic Shock to the Blood cells ( leukocytes). By adding sucrose to the solution, it absorbs (osmosis) glucose from the cell, hence cell is shrinked and ruptured. so nucleus is available for extraction. (By Nawaz Naji)
Focusing material is DNA, To extract the DNA from Human, BLOOD must be in preserved form and must not be coagulated so as to perform subsequent extraction steps. So to prevent Blood Clotting EDTA is used. It behaves as chelating agent for divalent ions (Ca++, Mg++). the question is that, why t…hese ions are needed to be captured. Simple is that Ca++ ions are initiator of Blood Clotting. and behave as cofactor of clotting enzymes, so it must be captured by EDTA. Hence EDTA prevents blood Coagulation( Clotting) by chelating these ions. Divalent ions are also co-factors of DNAase enzymes (that degrade DNA). so EDTA also prevents DNAase activity by chelating ions. (Nawaz Naji) (MORE)
To concentrate or purify the DNA, which is insoluble in isopropanol. Once the solution containing your DNA is placed in isopropanol and centrifuged, the DNA will precipitate to a little pellet at the bottom of your tube. Everything else in your tube is soluble in isopropanol and will remain in liqui…d form. Pipet the liquid out and now you have just DNA. (MORE)
Salt would attract the phosphate ends of DNA, therefore it pulls it way from other substances in the sample (Separation of DNA from surroundings)
Baking soda keeps the PH levels of the solution at an even level as the DNA is extracted.
Shampoo is a detergent, so it dissolves the cell membranes, allowing access to the DNA inside the cell.
This is to give a period of time for the DNA to grow by replication; this allows there to be enough of a sample of DNA to extract.
There are instructions to follow when you select a sample from your item bag. Follow them carefully and don't spill anything. When moving the X-ray screen to the Developer, move only the screen, not the whole tray. (for other notes, see the related question)
We can not extract DNA from RBCs as they are without nucleus. only the source of DNA extraction is Leukocytes, RBCs are not good source of extraction but we can extract DNA from immature RBCs.
some enzyme has degradation of cell membrane &also degrade protein & lipid of the cell....
You go to the grocery store and buy it. It comes in a small bottle. You can't make it at home unless you have a chemisty lab in your kitchen.
Most grocery stores carry banana extract. Some shops that sell cake decorating/baking supplies also sell a variety of extracts. It is also available through online shops that sell the same.
Yes! Just like all living things, bananas have DNA. It is also possible to extract DNA from a banana. There are tons of online labs to do this.
Maybe because bananas are annual kind of plant, which means they can survive in all year round in both rainy season and summer season and it's easy to produce unlike to the other plant to our surroundings. On the other hand, bananas have a juicy stem or body, it has a complete structures of genetic …information and besides banana can reproduce itself, without mating from the other plant nor from the other species of banana.(asexual reproduction). (MORE)
Single moleculesof DNA are long and stringy. Each cell of your body contains sixfeet of DNA, but it's only one-millionth of an inch wide. To fitall of this DNA into your cells, it needs to be packed efficiently.To solve this problem, DNA twists tightly and clumps togetherinside cells. Even when you …extract DNA from cells, it still clumpstogether, though not as much as it would inside the cell. (MORE)
blend the watermelon until to break the cell walls, add water if necessary. in a glass, mix 1 tbsp of liquid soap and 2 pinches of salt. the soap will break down the nucleus membrane that encapsulates the DNA. the salt will give an ionic condition necessary (will give positive charge that will neutr…alize DNA charge). be careful when you mix the soap and salt, you want least amount of bubbles. add 3 tbsp of the watermelon pulp to the soap mixture, and again, mix carefully because mixing vigorously will break the DNA fragments. filter the mixture with coffee filter. take 5 ml of the filtrate (the liquid, not the pulp in the filter cloth) and using a pipette, drop it to 5 ml of cold ethanol (or isopropanol) 1:1 ratio, if your watermelon is to watery, add more of it. do not mix it, let it sit for some minutes and it will coagulate. the DNA will rise to the top of the ethanol. (MORE)
One can get his DNA extracted at a crime laboratory, at a police precinct, at a hospital, and even at home. DNA can be extracted by using a cotton swab in the inside of one's cheek or through a blood sample.
Detergent is added in DNA extraction to breakdown and emulsify fatand proteins of the cell membrane.
In preparation for the electrophoresis step in "DNA fingerprinting"the electrophoresis process cannot separate meaningfully massivemolecules like whole chromosomes. By using restriction enzymes thatbreak the chromosomes at known places DNA fragments of a widevariety of lengths that the electrophores…is process can separatemeaningfully will allow a pattern to be generated that can identifydifferent individuals. (MORE)