A phase-contrast microscope (or phase-difference microscope or phase microscope) allows better viewing of clear specimens that usually need to be stained before you can make out the various parts. Staining is a time consuming process and kills the cells being viewed. With a phase-contrast microscope, living cells can be studied as they go through the cell division cycle.
HOW IT WORKS
As light goes through a transparent specimen, there may not be anything interesting to see. However, the light undergoes phase changes as it passes through the different parts of the specimen. Unfortunately, the human eye does not notice phase changes. A phase-contrast microscope converts these unnoticed phase changes into differences in brightness, which the human eye sees very well. The darker and lighter parts of the specimen make it very visible.
This also works on reflected light microscopes.
A phase microscope is also called a phase-contrast microscope or a phase-difference microscope.
A phase microscope allows better viewing of clear specimens which usually need to be stained before you can make out the various parts. Staining is a time consuming process and kills the cells being viewed. With a phase microscope, living cells can be studied as they go through the cell division cycle.
HOW IT WORKS
As light goes through a transparent specimen, there may not be anything interesting to see. However, there are phase changes to the light as it goes through the different parts of the specimen. Unfortunately, the human eye does not notice phase changes. A phase microscope converts these unnoticed phase changes into differences in brightness, which the human eye sees very well. The darker and lighter parts of the specimen make it very visible.
This also works on reflected light microscopes.
Its like a regular microscope but with the contrast up real high and upside down.
It accentuates phase changes produced in the specimen.
phase contrast
halo formation
The microscope that is used to see internal structures of cells in a natural state is the compound light microscope. The microscope has a lens and light that allows it to see the internal structures of a cell clearly.
phase contrast
telophase. in this phase the chromosome disperse and you can't see the chromosome anymore throught the light microscope.
diaphragm with annular stop and phase plate in the objective lens
Fritts Zernike invented the phase contrast microscope in 1932. He received a Nobel Prize for it in 1953.
The microscope that takes advantage of differences in the refractive indexes of cell structure is the phase contrast microscope. The microscope that is used to see internal structure of cells in a natural state is also a phase contrast microscope.
A phase microscope is also a contrast microscope. It empasizes slight differences in a specimen's capacity to bend light waves,enhancing light and darker regions without the use of stains.
Stereomicroscope, Compound Microscope, Phase-contrast microscope, electron microscope, Scanning-electron microscope, Transmission electron microscope, Confocal-scanning microscope. THESE ARE JUST SOME. :)
There are six different types of microscopes used in the life science. There is the light microscope, phase contrast microscope, fluorescent microscope, electron microscope, atomic force microscope, and scanning tunnelling microscope.
Frits Zernike
phase contrast
halo formation
The microscope that is used to see internal structures of cells in a natural state is the compound light microscope. The microscope has a lens and light that allows it to see the internal structures of a cell clearly.
In 1932
A phase-difference microscope (or phase-contrast microscope or phase microscope) allows better viewing of clear specimens that usually need to be stained before you can make out the various parts. Staining is a time consuming process and kills the cells being viewed. With a phase-difference microscope, living cells can be studied as they go through the cell division cycle.HOW IT WORKSAs light goes through a transparent specimen, there may not be anything interesting to see. However, the light undergoes phase changes as it passes through the different parts of the specimen. Unfortunately, the human eye does not notice phase changes.A phase-difference microscope converts these unnoticed phase changes into differences in brightness, which the human eye sees very well. The darker and lighter parts of the specimen make it very visible - and it is still alive!This also works on reflected light microscopes.