0
MacConkey agar is a selective medium designed to grow Gram negavite bacteria and stain them for lactose fermentation. It contains bile salts[to inhibit most Gram negative bacteria and some species of Staphlococcus], crystal violet dye, neutral red dye ,lactose and peptone. It was first developed by Alfred Theodore MacConkey. It is used to distinguish between lactose fermenting bacteria and non lactose fermenting bacteria. By utilising the lactose available in the medium, Lac+ bacteria such as Escherichia coli, Enterobacter and Klebsiella will produce acid, which lowers the pH of the agar below 6.8 and results in the appearance of red/pink colonies. Non-Lactose fermenting bacteria such as Salmonella, Proteus species and Shigella cannot utilize lactose, and will use peptone instead. This forms ammonia, which raises the pH of the agar, and leads to the formation of white/colorless colonies
Wiki User
∙ 15y ago
Add your answer:
Earn +20 pts
Is there a microorganism that degrades Agar agar?
Yes! Paenibacillus spp. can degrade agar agar
Can you elimintate gram positive bacteria from a gram positive gram negative mixture with mild detergent treatment?
AnswerYou can use a "selective" medium that will inhibit the growth of Gram positive bacteria and only allow Gram negative bacteria to multiply. A medium which is commonly used for this is the McConkey agar which contains a crystal violet strain and allows only Gram negative cultures to grow.You can also eliminate Gram positive bacteria with antibiotics (e.g. ampicillin) provided that they are sensitive and not resistant.
How many teaspoons is 7 g agar agar powder?
I teaspoon Agar Agar = 2 grams
From which organism agar agar is extracted?
red algae Agar agar is a malay term meaning red algae. This algae which agar is extracted lives in the ocean around Malaysia
Does micrococcus luteus grow on macconkey agar?
MacConkey agar is designed to grow Gram-negative bacteria and also contains crystal violet dye which inhibits the growth of Gram-positive bacteria. Since S. aureus is Gram-positive it should not grow on MacConkey agar. No. MacConkey agar inhibits MOST of the Gram-positive bacteria. There are some Gram-positive bacteria that can tolerate bile-salt are able to grow in MacConkey agar. Enterococcus and some species of Staphylococcus are able to grow in MacConkey. (lack citation though)
Why won't campylobacter jejuni grow on macconkey agar?
campylobacter can growth in mcconkey agar.
What is the birth name of Phil McConkey?
Phil McConkey's birth name is Philip Joseph McConkey.
What actors and actresses appeared in McConkey - 2013?
The cast of McConkey - 2013 includes: Bob Burnquist as himself Miles Daisher as himself Kent Kreitler Kent Kreitley Glenn McConkey Sherry McConkey Glenn McConkey as herself Sherry McConkey as herself Shane McConkey as himself Jonny Moseley as himself Travis Pastrana as himself Scot Schmidt
When was Mary McConkey born?
Mary McConkey was born in 1916.
When was Tony McConkey born?
Tony McConkey was born in 1963.
When was Bob McConkey born?
Bob McConkey was born in 1895.
When was Shane McConkey born?
Shane McConkey was born on 1969-12-30.
When was Thomas David McConkey born?
Thomas David McConkey was born in 1815.
When did Thomas David McConkey die?
Thomas David McConkey died in 1890.
When did Shane McConkey die?
Shane McConkey died on 2009-03-26.
What is Mcconkey agar media?
It's a differential agar used to differentiate between lactose fermenters and non-lactose fermenteing bacteria. Chemical composition of Mac Conkey agar Peptone 20.0g Trehalose 10.0g Bile salts(Difco) 1.5g Sodium Cloride 5.0 Neutral Red 0.05g Crystal Violet 0.001 Agar 15.0g Distelled water 1.0 liter pH: 7.4±0.2 (25 °C)
How tall is Phil McConkey?
Phil McConkey is 5' 10".
