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Restriction buffer maintains the pH in a range suitable for enzyme activity, as well as supplying salt cofactors required for catalysis. Since different restriction enzymes require varying salt conditions and pH, a single compromise buffer can be used that strikes a balance between conditions preferred by the various restriction enzymes. (Spec. compromise restriction buffer)
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∙ 15y ago
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∙ 14y agoEthidium bromide (EtBr) has two properties that are valuable for visualizing DNA. The first is that it binds to DNA. As the DNA fragments move through the gel during electrophoresis, the pick the EtBr from the gel. EtBr is also a flourescent dye. This allows us to view the position of the DNA fragments by placing the gel on a UV transilluminator.
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∙ 10y agoTBE (Tris Borate EDTA) buffer acts as an electrolyte to carry current through the gel, providing the motive force. Tris and borate are buffer salts, for pH stabilization. EDTA protect DNA against DNases, it is a chelating of divalent cations (Mg2+...) which are cofateurs of DNases.
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∙ 13y agoRestriction buffer maintains the pH in a range suitable for enzyme activity, as well as supplying salt cofactors required for catalysis. Since different restriction enzymes require varying salt conditions and pH, a single compromise buffer can be used that strikes a balance between conditions preferred by the various restriction enzymes. (Spec. compromise restriction buffer)
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∙ 10y ago"They cut the DNA into small fragments."
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∙ 10y agoTBE buffer in gel electrophoresis is used to maintain pH of te solution and prevents the denaturation of smale fragments of DNA.
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What is the function of the gel electrophoresis?
Gel electrophoresis is an analytical method used to separate DNA, RNA or proteins based on size
What is the buffer solution and why is it added to the agarose?
You use a buffer when making agarose gels so that when the gel is used for electrophoresis, the gel is able to conduct electricity. The buffer contains ions from the buffer salts that will facilitate conduction. that was good
What is the purpose of gel electrophoresis?
The buffer is the medium through which the current flows. In the electrophoresis chamber, the anode and cathode are separated and the gel is placed between them. In order to close the circuit and generate the voltage which causes the migration, the entire chamber is filled with a conductive buffer. It is actually possible to perform electrophoresis without a buffer; however this requires a specially made electrophoresis chamber. In these chambers the electrodes actually contact the top and bottom of the gel eliminating the need for a conductive buffer to close the circuit.SDS PAGE electrophoresis uses buffer not primarily as a conductor but for holding a desired pH, dissipating heat and providing SDS in excess in the case of denaturing gels. A gel would run without a buffer as the gel itself is a conductor but the currents involved would heat it to the point of decomposition. Also the volume of liquid in a gel does not allow for an adequate pH buffering system. Holding a pH is extremely important for reproducibility especially in native gels as the pH can change the charge on the peptide. It is true some gels do not require buffer but these are rare cases like isoelectric focusing.the primary application of the buffer would be to conduct electricity,to form a closed circuit
Why is a buffer solution need for electrophoresis?
Electrophoresis refers to the movement of charged particles in a fluid or gel under the influence of an electric field. It is carried out in buffer in order to complete the electron circuit flow.
Can a gel electrophoresis be performed both horizonnally and vertically?
yes for example 2D gel electrophoresis
What is the function of buffer in the gel electrophoresis?
TBE buffer in gel electrophoresis is used to maintain pH of te solution and prevents the denaturation of smale fragments of DNA.
What is the function of the gel electrophoresis?
Gel electrophoresis is an analytical method used to separate DNA, RNA or proteins based on size
What is used to sort DNA into different lenghts?
Gel Electrophoresis
What is the buffer solution and why is it added to the agarose?
You use a buffer when making agarose gels so that when the gel is used for electrophoresis, the gel is able to conduct electricity. The buffer contains ions from the buffer salts that will facilitate conduction. that was good
What is the purpose of gel electrophoresis?
The buffer is the medium through which the current flows. In the electrophoresis chamber, the anode and cathode are separated and the gel is placed between them. In order to close the circuit and generate the voltage which causes the migration, the entire chamber is filled with a conductive buffer. It is actually possible to perform electrophoresis without a buffer; however this requires a specially made electrophoresis chamber. In these chambers the electrodes actually contact the top and bottom of the gel eliminating the need for a conductive buffer to close the circuit.SDS PAGE electrophoresis uses buffer not primarily as a conductor but for holding a desired pH, dissipating heat and providing SDS in excess in the case of denaturing gels. A gel would run without a buffer as the gel itself is a conductor but the currents involved would heat it to the point of decomposition. Also the volume of liquid in a gel does not allow for an adequate pH buffering system. Holding a pH is extremely important for reproducibility especially in native gels as the pH can change the charge on the peptide. It is true some gels do not require buffer but these are rare cases like isoelectric focusing.the primary application of the buffer would be to conduct electricity,to form a closed circuit
Why is a buffer solution need for electrophoresis?
Electrophoresis refers to the movement of charged particles in a fluid or gel under the influence of an electric field. It is carried out in buffer in order to complete the electron circuit flow.
what is the gel in Gel Electrophoresis?
For larger molecules like proteins we use polyacrylamide gel electrophoresis (PAGE). For smaller pieces like DNA we use agarose gel electrophoresis
Can a gel electrophoresis be performed both horizonnally and vertically?
yes for example 2D gel electrophoresis
A technique that can be used to compare the DNA of two or more plants is?
Gel electrophoresis
What is a common name for gel electrophoresis?
gel
Where can one learn more on gel electrophoresis?
To learn more about gel electrophoresis, one can Google it. There is also a whole Wikipedia article dedicated to gel electrophoresis, and it happens to be quite informative.
What has the author A J Houtsmuller written?
A. J. Houtsmuller has written: 'Agarose-gel-electrophoresis of lipoproteins' -- subject(s): Blood protein electrophoresis, Electrophoresis, Gel electrophoresis, Lipoproteins
