PCR products produce million copies of your gene of interest. After PCR, we usually resolve them on the agarose gel to visualize the amplified DNA using EtBr stain under UV. The main purpose is, it make sure your gene is really amplified and the length it run is corresponding to the right size of your gene of interest and purify it from other template DNA and other unspecifically amplified DNA products by extracting from the gel.
Gel Electrophoresis
The ability to separate compounds sufficiently by their electric charge.
what kind of controls are run in the expirement? why are they important? could others be used? (PCR lab)
The migration of DNA/Protein on the gel (agarose/polyacrylamide) by the influence of electric charge is called gel electrophoresis. It is used to resolve the biomolecules according to their size(mainly) and shape(for proteins)
For larger molecules like proteins we use polyacrylamide gel electrophoresis (PAGE). For smaller pieces like DNA we use agarose gel electrophoresis
Pcr
Gel Electrophoresis
The ability to separate compounds sufficiently by their electric charge.
I think you should increase the annealing temperature and also reduce the PCR cycles.
what kind of controls are run in the expirement? why are they important? could others be used? (PCR lab)
The larger fragements will not be very accurate because they cannot resolve in high consentrations of the agarose in the gel. The percent of agarose in the gel affects the ability to resolve larger fragements of DNA
The migration of DNA/Protein on the gel (agarose/polyacrylamide) by the influence of electric charge is called gel electrophoresis. It is used to resolve the biomolecules according to their size(mainly) and shape(for proteins)
For larger molecules like proteins we use polyacrylamide gel electrophoresis (PAGE). For smaller pieces like DNA we use agarose gel electrophoresis
PCR or polymerase chain reaction. :: Apex
yes for example 2D gel electrophoresis
Gel electrophoresis
gel