The Rna triplet codon GUA, Thymine being replaced by Uracil in all Rna's.
The mRNA codon that would be complementary to the DNA codon CGT is GCA.
CUGUUCAGGUGUUAG
cua ggc
GCU aga
uac-gca
UACGCA
Cua ggc
That mRNA sequence had to come from the complement to it. Remeber that the sequence is normally read 5' to 3'. The complement that produced it would be seen in the 3' to 5' orientation (reverse) during transcription. Therefore, find the complement source by reading the sequence in reverse and making the following substitutions: a becomes t, u becomes a, g becomes c, and c becomes g. The result is the following DNA source sequence read 5' to 3': ctaagtcgcaatttttggcat.
If TACGTT is read 5'-TACGTT-3' then the complimentary strand will read 5'-AACGTA-3'. Since the template strand is traditionally written in the 5' to 3' direction then the complimentary strand, written in the same manner, would be AACGTA not ATGCAA. The four bases, adenine(A), thyamine(T), cytosin(C), and guanine(G) bond together in pairs. A - T, and C - G. They do not pair with any other base unless in the case of RNA, when thyamine is replaced with uracil.
Antisense technology is based on stopping the production of a protein. In order for a protein to be formed, the mRNA has to be translated (read by the ribosomes). The happens in the cytoplasm. There are stretches of nucleic acid bases called cDNA that are complimentary to specific mRNA sequences. These cDNA molecules can bind to the mRNA molecules and inactivate them. When mRNA is inactivated, it cannot be translated into a protein and a protein will not be formed. Since every protein is made by a certain mRNA, by inactivating the mRNA using cDNA molecules, the production of the protein can be stopped. This process is called mRNA silencing or antisense technology
In Translation, rRNA, tRNA, and mRNA are used. The mRNA strand of codons (3 nucleotide bases) enters the ribosome where the rRNA will "read it" (it is made up of rRNA). tRNAs are floating in the cell carrying the anticodon's specified amino acid. When the rRNA "sees" which codon is on the mRNA strand, the tRNA's anticodon (complementary nucleotide bases) binds to the A site in the ribosome to start creating the polypeptide chain.
Because Cytosine attaches with Guanine and adenine attaches with thymine, if CTA (cytosine, thymine, adenine) had another strand of DNA it should read GAT however mutations can occur.
The messenger RNA (or mRNA) compliment to a DNA strand of CCAGTT would be GGUCAA.
You would get ANRm.
The difference between the coding strand and the template strand is the coding strand is the strand which contains the coding genes, i.e. the one in which the RNA polymerase reads and transcribes into mRNA. It must have the promoter sequence in the correct orientation for transcription, as follows:5`-TATAATGCGCGCGCGCGCGCGCGC-3`3`-ATATTACGCGCGCGCGCGCGCGCG-5`In this sequence, the top strand is the coding strand, because it contains the promoter (TATAAT) in the correct orientation.However, when transcribed, the mRNA will be as follows:5`-GCGCGCGCGCGCGCGCGCGC-3`This is because the polymerase transcribes from the template strand, on the opposide side to the coding strand, to make it in the same orientation as the coding strand.I hope I have explained it enough for people to understand, however if I haven't please read this article I found which explains it thoroughly:http://www.bio.net/bionet/mm/bioforum/1994-May/008821.html
That mRNA sequence had to come from the complement to it. Remeber that the sequence is normally read 5' to 3'. The complement that produced it would be seen in the 3' to 5' orientation (reverse) during transcription. Therefore, find the complement source by reading the sequence in reverse and making the following substitutions: a becomes t, u becomes a, g becomes c, and c becomes g. The result is the following DNA source sequence read 5' to 3': ctaagtcgcaatttttggcat.
If TACGTT is read 5'-TACGTT-3' then the complimentary strand will read 5'-AACGTA-3'. Since the template strand is traditionally written in the 5' to 3' direction then the complimentary strand, written in the same manner, would be AACGTA not ATGCAA. The four bases, adenine(A), thyamine(T), cytosin(C), and guanine(G) bond together in pairs. A - T, and C - G. They do not pair with any other base unless in the case of RNA, when thyamine is replaced with uracil.
The template strand (DNA) is read by RNA polymerase in the 3'-5' direction. First, RNA polymerase binds to an A-T rich promoter on the DNA which is upstream from the site of translation. Because A-T bonds are weaker than C-G bonds, the double helix opens up at this point and RNA polymerase begins translation. While translaing the DNA template strand, it creates a complementory mRNA strand and thus the Mrna will read 5'-3' with new bases being added at the 3' end.
The plus strand is the same as the sense strand and can also be called the coding or non-template strand. This is the strand that has the same sequence as the mRNA (except it has Ts instead of Us). The other strand, called the template, minus, or antisense strand, is complementary to the mRNA. Gotta love the use of 4 names to describe the same thing. Ah science, why do you torment us?
If a DNA strand read CCTAGCT, its mRNA would read GGAUCGA.
Antisense technology is based on stopping the production of a protein. In order for a protein to be formed, the mRNA has to be translated (read by the ribosomes). The happens in the cytoplasm. There are stretches of nucleic acid bases called cDNA that are complimentary to specific mRNA sequences. These cDNA molecules can bind to the mRNA molecules and inactivate them. When mRNA is inactivated, it cannot be translated into a protein and a protein will not be formed. Since every protein is made by a certain mRNA, by inactivating the mRNA using cDNA molecules, the production of the protein can be stopped. This process is called mRNA silencing or antisense technology
This has to be a strand of DNA because RNA does not have Thymine (T), instead it has Uracil (U).Thus, if this strand were RNA it would read:5' augcuaucauugaccuugaguuauuaa 3'
The messenger RNA strand. When the tRNA inserts itself between the two portions of the ribosome attached to the mRNA strand, the specific tRNA depends on the 3 nitrogen bases on the mRNA (the codon) that are about to be read. The tRNA that arrives has a corresponding "anticodon" to go with the codon on the mRNA. For example, if the nitrogen bases on the mRNA strand are adenine, guanine, and cytocine the tRNA will have an anticodon of uracil, cytocine and guanine. The tRNA that has the corresponding anticodon to the codon on the mRNA will bring with it a specific amino acid but it is the codon on the mRNA that ultimately decided which amino acid is next in line.
If a mutation affects the mRNA, then the sequence of amino acids that it codes for may be incorrect, causing the protein produced by the amino acids to not function properly, or it may not function at all.