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A certain restriction enzyme digest results in DNA fragments What sizes 4000 base pairs 2500 base pairs 2000 base pairs 400 base pairs?
Wiki User
∙ 13y ago
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(the amount of strands represents the size of the DNA strands)
Wiki User
∙ 13y ago
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What is meant by the term restriction digest?
Its the process of cutting DNA molecules into smaller pieces with special enzymes called Restriction Endonucleases (sometimes just called Restriction Enzymes or RE's).
Can protease digest amino acid?
No, proteases digest proteins, producing protein fragments. The smallest fragment of a protein is an amino acid, the monomers from which the polymers we call proteins are built.
How many lambda DNA fragments does BamHI make?
There are five BamHI cut sites in lambda DNA: Location 5505 22346 27972 34499 41732 So, BamHI will digest lambda DNA into six fragments.
What is a fusion primer?
Not 100% sure, but I believe it's a primer with a linker attached to it. A linker is a short DNA that has a restriction site built-in. Imagine this: you want to amplify a gene by PCR, but it has no suitable restriction site for you to clone into a vector. So by PCR with a fusion primer (with built-in restriction site), you can simply digest the ends and ligate it into your vector
Does the parasympathetic nervous system increase or decrease BP?
The parasympathetic nervous system serves as the "rest and digest" system, which results in vasodilation of the peripheral vessels. This results in an overall decrease in blood pressure :)
What is meant by the term restriction digest?
Its the process of cutting DNA molecules into smaller pieces with special enzymes called Restriction Endonucleases (sometimes just called Restriction Enzymes or RE's).
Can protease digest amino acid?
No, proteases digest proteins, producing protein fragments. The smallest fragment of a protein is an amino acid, the monomers from which the polymers we call proteins are built.
What are you trying to get out restriction digest?
you are trying to get out pop and making more space for your body so it will have more food to storage
How many lambda DNA fragments does BamHI make?
There are five BamHI cut sites in lambda DNA: Location 5505 22346 27972 34499 41732 So, BamHI will digest lambda DNA into six fragments.
When something Has certain features to help it do a certain job?
an organ used to digest and absorb food
What is a fusion primer?
Not 100% sure, but I believe it's a primer with a linker attached to it. A linker is a short DNA that has a restriction site built-in. Imagine this: you want to amplify a gene by PCR, but it has no suitable restriction site for you to clone into a vector. So by PCR with a fusion primer (with built-in restriction site), you can simply digest the ends and ligate it into your vector
What foods does the body have a hard time digesting?
Your body finds it hard to digest corn (on the cob) and that results in corn bits coming out when you do your "business". It helps with keeping your digest working because it is trying to digest that corn.
Describe the difference between a single digest and a double digest?
Restriction enzymes (endonucleases) are used for a variety of reasons in molecular genetics, including obtaining a "map" and cloning DNA. Single digests consitute DNA being treated with one restriction endonuclease, whereas double digests contain 2 enzymes. At times, it is difficult (or not possible) to perform double digests ... especially when the 2 enzymes have very different requirements for their activities (e.g. salt concentration, temperature optimums, ...). If a DNA restriction map is known for a particular enzyme, and if the DNA is treated with this enzyme, then one can ascertain whether the digest was complete or not. However, if a restrictioin map is just being compiled, and if the DNA is treated with 2 enzymes in a double digest, at times difficulties may arise in determining the map if either (or both) enzymes did not completely digest the DNA.
Why is it helpful to digest each of your samples with two different restriction enzymes?
It helps break up your sample even more. Your first enzyme may, for example, ONLY cut at a sequence of TAATTA ---> TA // ATTA. Maybe your second enzyme is less selective, and will cut ANY GC --> G // C. Using them together, you will end up with much smaller fragments than using only enzyme #1 for example.
What are processes in digestion of foods?
Foods are digested differently for certain people. Typically, pepsin from the stomach is used to digest proteins, and amylase from the liver is used to digest carbohydrates.
Can you tell me about eating for your blood type?
You eat for your blood type be eating certain foods that respond the best with your metabolism and digestive system. Certain blood types digest certain foods differently.
Which Restriction enzyme are studied in Recombinant DNA Technology?
It's not the restriction enzymes that are studied, its the DNA. The enzyme cuts or "restricts" the DNA strand at a known sequence of nucleotides. Different enzyme, different sequence. For a Biomanufacturing application, where we want to insert foreign DNA, the gene of interest is cut and spliced with a restriction enzyme into a recombinant plasmid, transformed into a bacteria, and sent merrily on it's way to make Insulin, or whatever. With an unknown piece of DNA (a functional gene that makes a protein of interest or is being studied), the plasmid has "restriction sites" or nucleotide sequences, for several restriction enzymes, all of which I have mapped out. The unknown piece of DNA is cut at each end by a single restriction enzyme and inserted into the plasmid, which gives me some landmarks. I insert the plasmid into a bacteria, grow a culture so the bacteria makes many millions of copies of the plasmid, extract the plasmid, and run an experiment called a restriction digest. The restriction digests are a series of reaction with single enzyme and combinations of two and three enzymes, all cutting the plasmid at different nucleotide sequences. Then I run an agarose gel electrophoresis, which separates all the different pieces of DNA by size, and do an analysis called a Restriction Map. This counts the DNA fragments and their sizes, which enzyme and combination of enzymes produced which sizes and how many fragments, which enzyme cuts where, which cuts were definitely in the known part of the plasmid, which were probably in the unknown DNA, adding up nucleotide sequence numbers to make sure different mapping guesses agree, etcetera, etcetera, and so forth. Until at last, a map of the size and restriction sites of the unknown DNA insert into the known plasmid vector is deduced. This used to be done by hand, but there are computer programs that do it now. This is Research, the Technology is down the line a few steps when the gene has been characterized, the protein produced has been characterized, the trials are done, and the restriction enzyme to insert the gene into the bacteria for Bioman has been established
