0
Why is it helpful to digest each of your samples with two different restriction enzymes?
Wiki User
∙ 11y ago
It helps break up your sample even more. Your first enzyme may, for example, ONLY cut at a sequence of TAATTA ---> TA // ATTA.
Maybe your second enzyme is less selective, and will cut ANY GC --> G // C.
Using them together, you will end up with much smaller fragments than using only enzyme #1 for example.
Wiki User
∙ 11y ago
Add your answer:
Earn +20 pts
DNA strands can be clipped crosswise at selected positions by using enzymes called?
DNA can be cut into smaller fragments by enzymes (which are proteins) known as restriction endonucleases (REN's). These enzymes are sequence specific - meaning they produce a cut only at a particular site on the DNA strand. This site where the cut is produced is called the restriction site. Restriction sites are 4 - 6 nucleotides in length. Every restriction enzyme has a different restriction site. This property allows researchers to treat two different DNA samples with the same set of restriction enzymes and then analyze the resulting fragments.A. DNA finger printing
What is maxam Gilbert method of DNA packaging?
Used in DNA sequencing; four samples of end-labeled DNA restriction fragments are chemically cleaved at different specific nucleotides. The resulting subfragments are separated by gel electrophoresis, and the labeled fragments are detected by autoradiography. The sequence of the original end-labeled restriction fragment can be determined directly from parallel electropherograms of the four samples
Why is it important to correctly construct the DNA model of the different samples?
Why is it important to correctly construct the DNA model of the different samples?
What is DNA Standard used for in a fingerprint?
The DNA strand of each organism has some sequence different from others, but there are some sequences which are known as "variable number tandem repeats" (VNTR), which are repeated many times in the DNA of an individual. These are basis of "DNA fingerprinting". DNA is cut with a restriction enzyme from the specific sites of these repeats. When the DNA of two samples are cut by these restriction enzymes, and run on gel electrophoresis, the band pattern can be matched or compared. Imagine what would happen if you got a sample of DNA from a crime scene and wanted to compare it with another sample, run years earlier or in another country. The gels would have been run at different times, by different people, under different conditions, so perhaps you could not be sure if the bands on the gel match or not. To solve this problem gels are run with both the samples and a reference material, which serves two purposes. Firstly it assures us that the gel has run correctly and that samples were treated correctly. Secondly it means we can determine what the bands of our test DNA mean by comparing them to the reference material. Sometimes the reference DNA is called the standard reference material (SRM) and we commonly used SRM2390 (for RFLP), SRM2391a (for PCR) and SRM 2392 (mitochondrial DNA) in DNA fingerprinting. So the skinny answer is that DNA standards are used to assure the quality and comparability of the test performed.
What is the difference between cheek cells seen under the microscope and those illustrated in text books?
The major difference between cheek cells seen under a microscope and those in illustrated text books are the samples used. Different samples will yield different results.?æ
Why are DNA samples and restriction enzymes incubated for 5 minutes?
uranes then myanes
DNA strands can be clipped crosswise at selected positions by using enzymes called?
DNA can be cut into smaller fragments by enzymes (which are proteins) known as restriction endonucleases (REN's). These enzymes are sequence specific - meaning they produce a cut only at a particular site on the DNA strand. This site where the cut is produced is called the restriction site. Restriction sites are 4 - 6 nucleotides in length. Every restriction enzyme has a different restriction site. This property allows researchers to treat two different DNA samples with the same set of restriction enzymes and then analyze the resulting fragments.A. DNA finger printing
Why is DNA fingerprinting more accurate if the samples are cut with more than one restriction enzyme?
When EcoR1 cuts this DNA, it cuts it at three places into four different segments. EcoR1 is only one of many different restriction enzymes. Each different enzyme cuts DNA at a different site. By using different enzymes, a scientist can cut DNA into many smaller pieces that can be run out on a gel during electrophoresis. Remember that in gel electrophoresis, DNA fragments separate by size. Because these segments have different sizes, they will separate onto a gel at different rates. If different people's DNA is cut by restriction enzymes and then run out on a gel, each person's DNA will leave a different pattern.
How are restriction enzymes used to look for differences between DNA samples?
Restriction enzymes cleave, or open, the DNA so that a sample can be taken and gel electrophoresis can separate the strands of DNA. From there, DNA probes bind to certain strands in each sample and DNA fingerprints can show the differences.
What is maxam Gilbert method of DNA packaging?
Used in DNA sequencing; four samples of end-labeled DNA restriction fragments are chemically cleaved at different specific nucleotides. The resulting subfragments are separated by gel electrophoresis, and the labeled fragments are detected by autoradiography. The sequence of the original end-labeled restriction fragment can be determined directly from parallel electropherograms of the four samples
How do you use electrophoresis to determine paternity of a baby?
Children receive half of their genetic material from each parent. There are specific sites on DNA, known as restriction sites, that are recognized by restriction enzymes. These are used to determine paternity. Samples of DNA from the mother, father and child are taken. They are all digested ('cut') by the same restriction enzymes. These DNA fragments are then separated by gel electrophoresis (which separates fragments based on size). The bands of the child are compared to the mother and father's. If the band is not the same as the mother's, it must have come from the father. If these do not match up, then the sample was not taken from the biological father.
Why do polls have different outcomes?
Because they are based on samples and outcomes vary between different samples.
How long are Blood samples good for testing?
Blood samples can be held in storage for 17 to 180 days. The length of their storage time depends on which enzymes have been added to the sample.
How many different simple random samples of 7 are possible from a pool of 25?
There are 25C7 different samples of seven from a pool of 25.25C7 = 25!/(7!(25-7)!) = 480 700 different samples of 7
Why should teachers provide students with resume samples?
Teachers should provide students with resume samples because these samples can be very helpful for students who are just starting out and haven't yet figured out to form a good resume.
Why is it important to correctly construct the DNA model of the different samples?
Why is it important to correctly construct the DNA model of the different samples?
Which is not a characteristic of a compound-?
Different samples of a compound do not have different properties.
