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Why is DNA fingerprinting more accurate if the samples are cut with more than one restriction enzyme?
When EcoR1 cuts this DNA, it cuts it at three places into four different segments. EcoR1 is only one of many different restriction enzymes. Each different enzyme cuts DNA at a different site. By using different enzymes, a scientist can cut DNA into many smaller pieces that can be run out on a gel during electrophoresis. Remember that in gel electrophoresis, DNA fragments separate by size.
Because these segments have different sizes, they will separate onto a gel at different rates. If different people's DNA is cut by restriction enzymes and then run out on a gel, each person's DNA
will leave a different pattern.
What else can I help you with?
What is the significance of the restriction enzyme GGATCC in molecular biology research?
The restriction enzyme GGATCC is significant in molecular biology research because it recognizes and cuts DNA at a specific sequence, allowing scientists to manipulate and study DNA molecules. This enzyme is commonly used in genetic engineering techniques such as gene cloning and DNA fingerprinting.
An enzyme that cuts double-stranded DNA at specific nucleotide sequences?
Such an enzyme is called a restriction endonuclease
Which restriction enzyme did you use to cut the DNA?
The restriction enzyme used to cut the DNA was EcoRI.
What happens when a restriction enzyme is used on DNA?
when restriction enzyme is use on DNA basically it just first losen up the DNA, usally DNA is coiled, and so the restriction enzyme jsut breka the DNA and leave a sticky end, so that it can be put back together, the cell have to be able to do that because in nature, that's the way for cell to stop protein production and the cell still need that gene
Hi does any one know if I want to put some restriction enzyme close to cDNA should I put some nucleotide befor this restriction enzyme?
Restriction enzyme cuts DNA strand at specific locations Restriction enzyme cuts DNA strand at specific locations
Restriction enzyme digestion?
Yes?
Where does the restriction enzyme EcoRI cut in a DNA sequence?
The restriction enzyme EcoRI cuts DNA at a specific sequence of bases, which is GAATTC.
What is the difference between a restriction enzyme and an endonuclease?
A restriction enzyme is a type of endonuclease. Endonucleases are enzymes that cut DNA at specific sequences, while restriction enzymes specifically cut DNA at recognition sites called restriction sites.
Where in the DNA sequence does the restriction enzyme EcoR1 specifically cut?
The restriction enzyme EcoR1 specifically cuts the DNA sequence at the recognition site GAATTC.
What determines how DNA will be cut by a restriction enzyme?
Restriction enzymes cut DNA at specific sites called restriction sites. These restriction sites are typically 6 - 8 nucleotides in length and have a defined set of nucleotide bases. For example, the restriction enzyme Eco R1 cuts at the site: AGGTTC. Therefore, if the target DNA contains the above sequence, Eco R1 is able to cut it within the restriction site. Hence, by looking into the target site and which restriction enzymes are being used, on can make an accurate estimate of where the target DNA will be cut
What enzyme is responsible for cutting DNA molecules?
The enzyme responsible for cutting DNA molecules is called a restriction enzyme.
What is the restriction site of the restriction enzyme TaqI?
TaqI's restriction site is:TCGAAGCT
