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They should not. Polysporin is an antibiotic, and while it may help clear up an infection, it should not have any anesthetic effect that would dilate the cat's pupils. That said, a cat's pupils dilate naturally when she is afraid, and it is likely that the process of putting polysporin drops in her eyes does alarm her for a little while.

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Q: Are polysporin eye drops making your cats eyes to deliate?
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