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it is nonpolar since C-H bonds are considered a nonpolar bond
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what is the difference c3 and c4 hplc column?
The difference is the length of the carbon alkyl chain off of the silica bead support in the column. The higher the number, the longer the chain, the more "reverse phase" it is. For example a polar compound is best separated on a C3 if you must use reverse phase and a non-polar compound will be best separated on a C18 column.
What is C8 and C18?
HPLC columns. (HPLC - High Performance Liquid Chromatography.)
Can you use a C18 column for HPLC with fluorescence detector and methanol as the mobile phase?
Yes, you can use a C18 column and methanol as a mobile phase with fluorescence detector. Fluorescence detector is generally used as it can detect the presence of compounds at a very low concentration.
How many types of hplc columns?
There are several column types, according to their function, they can be classified as: a) Normal phase In this column type, the retention is governed by the interaction of the polar parts of the stationary phase and solute. For retention to occur in normal phase, the packing must be more polar than the mobile phase with respect to the sample. Therefore, the stationary phase is usually silica and typical mobile phases are hexane, methylene chloride, chloroform, diethyl ether, and mixtures of these. b) Reverse phase In this column the packing material is relatively nonpolar and the solvent is polar with respect to the sample. Retention is the result of the interaction of the nonpolar components of the solutes and the nonpolar stationary phase. Typical stationary phases are nonpolar hydrocarbons, waxy liquids, or bonded hydrocarbons (such as C18, C8, etc.) and the solvents are polar aqueous-organic mixtures such as methanol-water or acetonitrile-water. c) Size exclusion In this column type, molecules are separated according to size. Small molecules penetrate into the pores within the packing while larger molecules only partially penetrate the pores. The large molecules elute before the smaller molecules. d) Ion exchange In this column type the sample components are separated based upon attractive ionic forces between molecules carrying charged groups of opposite charge to those charges on the stationary phase. Separations are made between a polar mobile liquid, usually water containing salts or small amounts of alcohols, and a stationary phase containing either acidic or basic fixed sites. This HP 1090 Chromatograph is also equipped with an oven in the column compartment. The function of the oven is to provide an homogeneus air-bath temperature when it is required for some methods, such as the carbohydrate separation method which requires a constant temperature of 85ºC.
How do you distinguised np-hplc and rp-hplc?
NP-HPLC is "Normal Phase" HPLC, wherein the solvents used are less polar than the substrate in the HPLC column (e.g. using hexane or dichloromethane with a silica HPLC column). RP-HPLC is "Reverse-Phase" HPLC, wherein the solvents used are more polar than the substrate in the HPLC column (e.g. using Water and Methanol with a octadecylsilane (ODS or C18) column).
What is C18 phase in hplc column?
Both C8 and C18 columns are considered as examples of reversed phase liquid chromatography (RP). The stationary phase here is seen as a thin film of non-polar liquid phase that has been designed to be chemically anchored to an inert material (Silica gel particles). The non-polar layer is chemically linked to the silica particles surface by reaction with the polar silanol groups on the stationary phase surface and so rendering them (less polar or non-polar), The difference between the two columns will be in the length of the carbon-chain attached to the silica surface. Acoordingly C8 hplc columns have packing material composed of silica particles attached to C8 carbon units while C18 will, of course, have packing materials coated with C18 hydrophobic units. Categorically both are reversed phase but C18 columns will definitely be more "hydrophobic rather than the C8 columns.
When you use c8 and when c18 column?
Both C8 and C18 columns are considered as examples of reversed phase liquid chromatography (RP). The stationary phase here is seen as a thin film of non-polar liquid phase that has been designed to be chemically anchored to an inert material (Silica gel particles). The non-polar layer is chemically linked to the silica particles surface by reaction with the polar silanol groups on the stationary phase surface and so rendering them (less polar or non-polar), The difference between the two columns will be in the length of the carbon-chain attached to the silica surface. Acoordingly C8 hplc columns have packing material composed of silica particles attached to C8 carbon units while C18 will, of course, have packing materials coated with C18 hydrophobic units. Categorically both are reversed phase but C18 columns will definitely be more "hydrophobic" rather than the C8 columns. See also the links below.
How do you select hplc column in method development?
firs you mist know the polarity for sample, wen the sample polar you can use "RP" column like C18 or C8 ( C18 first in pharmaceutical) . wen sample non polar use "NP" column like silica or CN Column. after that you can change the column in same packing to solve tailing, retention time, Resolution..... or any problem by change column length, particle size or carbon loud
Is diesel saturated or unsaturated hydrocarbon?
Kerosene or Kerosine is neither. It neither belongs under the Aliphatic or Aromatic branch of Organic chemistry. It's a different branch of Chemistry called Hydrocarbon Derivatives (which contain other organic compounds such as alcohol, ethers and amides). Kerosine fits under a different branch of organic chemistry.Aliphatic hydrocarbons are compounds in organic chemistry that consist of carbon and hydrogen and are non-aromatic.
Impact of Octadecanoylcarnitine C18 deficiency in children?
How can it raised
What is the sum(c5c18) in cell c19 in excel?
=SUM(C5:C18) will add the values in all the cells from C5 to C18 and put the total in C19 when the formula is placed there.
what is the difference c3 and c4 hplc column?
The difference is the length of the carbon alkyl chain off of the silica bead support in the column. The higher the number, the longer the chain, the more "reverse phase" it is. For example a polar compound is best separated on a C3 if you must use reverse phase and a non-polar compound will be best separated on a C18 column.
What is C8 and C18?
HPLC columns. (HPLC - High Performance Liquid Chromatography.)
What is the common name of a fatty acid that has c18 2n3 structure?
Alpha Linoleic acid
What nationality is the surname Cattee?
French, they were Huguenots, many of whom escaped from persecution in the c18
Can you use a C18 column for HPLC with fluorescence detector and methanol as the mobile phase?
Yes, you can use a C18 column and methanol as a mobile phase with fluorescence detector. Fluorescence detector is generally used as it can detect the presence of compounds at a very low concentration.
How many types of hplc columns?
There are several column types, according to their function, they can be classified as: a) Normal phase In this column type, the retention is governed by the interaction of the polar parts of the stationary phase and solute. For retention to occur in normal phase, the packing must be more polar than the mobile phase with respect to the sample. Therefore, the stationary phase is usually silica and typical mobile phases are hexane, methylene chloride, chloroform, diethyl ether, and mixtures of these. b) Reverse phase In this column the packing material is relatively nonpolar and the solvent is polar with respect to the sample. Retention is the result of the interaction of the nonpolar components of the solutes and the nonpolar stationary phase. Typical stationary phases are nonpolar hydrocarbons, waxy liquids, or bonded hydrocarbons (such as C18, C8, etc.) and the solvents are polar aqueous-organic mixtures such as methanol-water or acetonitrile-water. c) Size exclusion In this column type, molecules are separated according to size. Small molecules penetrate into the pores within the packing while larger molecules only partially penetrate the pores. The large molecules elute before the smaller molecules. d) Ion exchange In this column type the sample components are separated based upon attractive ionic forces between molecules carrying charged groups of opposite charge to those charges on the stationary phase. Separations are made between a polar mobile liquid, usually water containing salts or small amounts of alcohols, and a stationary phase containing either acidic or basic fixed sites. This HP 1090 Chromatograph is also equipped with an oven in the column compartment. The function of the oven is to provide an homogeneus air-bath temperature when it is required for some methods, such as the carbohydrate separation method which requires a constant temperature of 85ºC.
