Crystal violet, basic fuchsin, and safranin are all dyes which can be used in direct staining because they are cationic which means that they are positively charged. These dyes which are positively charged will react to the bacterial cell wall because the cell wall is negatively charged resulting in a basic stain.
can dyes other than methylene blue be used for direct staining?
There are other dyes such as Safranin, and Crystal Violet that may be used.
In some instances, such as in Zieh-Neelsen staining, you can substitute malachite green for methylene blue in the counter staining step.
Methylene blue isn't even used in Gram staining, so there's one counterexample for you.
No,yes,no,yes,no,yes
Yes
Adding methylene blue to a slide will stain animal cells and make the nuclei more visible.
iodine
There are a few staining methods that can be used. Common are methylene blue, ethidium bromide, fast blast, sybr safe.
Eosin is a red stand and methylene blue is blue. The result of staining a bacterial smear with a mixture of eosin and methylene blue is that eosin is acidic and acts as a negative stain. Methylene blue is basic the smear background would turn out red while the cells would turn out blue.
Methylene blue is used for many different staining purposes, but one of the main ones is staining RNA or DNA. In animal cells, it will stain the cytoplasm and the nucleus (the nucleus will be much darker).
Methylene blue stains everything blue.
blue bacilli
Adding methylene blue to a slide will stain animal cells and make the nuclei more visible.
the ph of the stain on the bacteria caused by methylene blue would not affect it a lot since all methylene blue is supposed to do is make it visible on the microscope for e.g.
iodine
no
Eosin and methylene blue are used for staining biolgical tissues.
There are a few staining methods that can be used. Common are methylene blue, ethidium bromide, fast blast, sybr safe.
Both are used in staining but for different purposes .
A secondary stain is Methylene blue. This type of stain is used in a acid fast staining. This type of staining test can determine medical conditions such as tuberculosis.
Eosin is a red stand and methylene blue is blue. The result of staining a bacterial smear with a mixture of eosin and methylene blue is that eosin is acidic and acts as a negative stain. Methylene blue is basic the smear background would turn out red while the cells would turn out blue.
Methylene blue can be used to stain E. coli so you can look at it under a microscope. As an alternative, you could try gram staining.