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A secondary stain is Methylene blue. This type of stain is used in a acid fast staining. This type of staining test can determine medical conditions such as tuberculosis.
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What is the secondary stain in gram stain procedure?
It is crystal violet & stains all cells purple.
What type of dye is used to stain the specimen when the acid-fast stain and the gram stain are used?
Both processes use 2 stains. The Gram staining process uses crystal violet as the primary stain and safranin as the secondary stain. Acid-fast staining uses carbol fuchsin as the primary and methylene blue as the secondary.
Is B subtilis a gram positive or gram negative bacterium?
gram positive Exactly. When doing a gram stain on B. subtilis, this bacterium resists decolorization (keping the first stain and NOT taking on the color of the secondary stain). Therefore, this bacterium is gram (+).
What kind of stain do you stain Mycobacterium with?
For Mycobacterium you will use the Acid-fast staining technique. There are two different methods of stainging: 1) Ziehl-Neelsen Method and 2) Kinyoun Method.1) The Ziel-Neelsen method uses a primary stain of Carbol Fuchsin dye that must be steam treated, rinsed with acid alcohol wash, and a secondary stain of Methylene Blue.2) The Kinyoun Method uses a primary stain of Kinyoun Carbol Fuchsin dye that is not steam treated. An acid alcohol wash is applied and a secondary dye of Brilliant Green. This technique is called "cold staining".The mycolic acid within the Mycobacterium cell membrane has a high affinity for the Carbol Fuchsin dyes.
What is the difference between Wright stain and Giemsa stain?
The difference between Wright Stain and Giemsa Stain is the intensity of the stain. The Giemsa Stain provides a better stain intensity than the Giemsa stain.
What is secondary and counter stain?
counterstains are selected to be contrasting color so that the target of the primary stain can easily be differentiated on a contrasting background. This makes life easier, when, for example you need to count the number of nuclei in a smear, or number of gram positive bacteria in a mixed population.
Can you stain on top of the old stain?
yes you can and the stain can change colors if there is a juice stain for example thats red and a grass stain the stain color could change
How could you describe a stain instead of saying a big stain what could you say?
a huge stain. a jumbo stain. a large stain.
Is maneval's stain an acid stain?
it is a basic dye that will stain the cells. That makes it a positive stain.
How do surfactants work in stain removers?
Surfectants work in stain removers to remove the stain and not damage what the stain is on. It oxidizes the stain and lifts it out of whatever its in,
How does stain remover work?
A stain remover may be a solvent which dissolve the stain or a substance which react with the stain.
Why does the counterstain not change the color of all of the cells is it because the primary stain repels or masks the secondary stain?
In a gram stain the primary stain is crystal violet. Iodine then sets that dye into the gram positive cells while alcohol washes out the crystal violet from the gram negative cells. Then safranin, which is the counterstain in a gram stain, is used to dye the rest of the bacteria. This is the example I can give you of why a counterstain does not change the look in all the cells. Though safranin stains all the cells, the gram positive cells that were dyed purple from crystal violet don't look pink - only the gram negative do.
