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There are a few staining methods that can be used. Common are methylene blue, ethidium bromide, fast blast, sybr safe.
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Why is staining the DNA an important step?
You can't see it if it's not stained.
Write up on mechanism of DNA precipitation?
DNA molecules are surrounded by water molecules when they are in solution. This is called the shell of hydration. When the salt concentration of the solution is increased, the sale molecules replace the water molecules in the shell of hydration causing the DNA to come out of solution. This 'coming out' of the DNA from a soluble form is called DNA precipitation. Precipitated DNA generally is surrounded by a lot of salt and has to therefore be placed in a low salt buffer in order to make sure it remains pure and integral (in an unbroken state)
Role of spooling in blood DNA isolation?
spooling of the DNA results from the addition of the ethanol which is insoluble in the solution. after we get he DNA in the form of spooling structure the solution is centrifuged. so we get the DNA.
What is the function in the following reagents used in the extraction and precipitation of DNA?
to extract the DNA from the solution, isopropyl alcohol is mixed with the DNA solution to precipitate the same.
Do gram staining divide bacteria into two groups based on difference in the composition of their DNA?
no. it's their cell walls.
What are the dark staining regions in the the nucleus of a non-dividing cell which are composed of tightly coiled strands of DNA called?
Condensed chromatin
Why is staining the DNA an important step?
You can't see it if it's not stained.
The dark staining material scattered within the nucleus has been shown to be?
dna
Write up on mechanism of DNA precipitation?
DNA molecules are surrounded by water molecules when they are in solution. This is called the shell of hydration. When the salt concentration of the solution is increased, the sale molecules replace the water molecules in the shell of hydration causing the DNA to come out of solution. This 'coming out' of the DNA from a soluble form is called DNA precipitation. Precipitated DNA generally is surrounded by a lot of salt and has to therefore be placed in a low salt buffer in order to make sure it remains pure and integral (in an unbroken state)
What is the difference between leishman staining and giemsa staining?
Leishman staining is used for staining blood in microscopy and its purpose is to both identify and differentiate trypanosomas, leucocytes and malaria parasites. Giesma staining is used to stain DNA region, specifically chromosomes in order to locate aberrations like rearrangement and translocations.
Role of spooling in blood DNA isolation?
spooling of the DNA results from the addition of the ethanol which is insoluble in the solution. after we get he DNA in the form of spooling structure the solution is centrifuged. so we get the DNA.
What is the function in the following reagents used in the extraction and precipitation of DNA?
to extract the DNA from the solution, isopropyl alcohol is mixed with the DNA solution to precipitate the same.
Cell structures that carry the genetic material that is copied and passed from generation to generation of cells?
Cell structures that carry the genetic material that is copied and passed from generation to generation of cells are called chromosomes. Spindles are cell structures composed of microtubule fibers.
What is the name of the test for the nucleic acid DNA in a solution?
What is the name of the test for the nucleic acid DNA in the solution.
What is the purpose to add ethanol in DNA solution?
To precipitate the DNA out of solution. It is usually done in the presence of salt, such as sodium chloride or potassium sulfate. This process is called "salting out", meaning becoming out of solution (water), which also can be done with other electrically charged molecules (ionized), including proteins.
What are darkly staining structures called?
chromosomes
What causes two strands of DNA to split?
The answer depends on the circumstances! In a cell, during DNA replication or during translation, the two strands in a DNA molecule are separated by enzymes called topoisomerases and helicases. In a solution, the two strands of a DNA molecule can be separated by being heated. This is called DNA melting. See: http://en.wikipedia.org/wiki/DNA http://en.wikipedia.org/wiki/DNA_melting
