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spooling of the DNA results from the addition of the ethanol which is insoluble in the solution. after we get he DNA in the form of spooling structure the solution is centrifuged. so we get the DNA.
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∙ 14y ago
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Role of sucrose in DNA isolation from human blood?
Sucrose performs the function of osmoregulation in the protocol of DNA isolation from blood
Role of mgcl2 in DNA isolation?
mgcl2 work as a cofactor and they enhance the enzymatic reaction..
What does ctab use for isolation of DNA from animal blood?
CTAB is a detergent used to denature proteins from samples. Once the protens have been denatures, the isolation of DNA from the non required waste materials can begin.
Use of agrose in DNA isolation?
Agarose is not used in DNA isolation. Agarose is used to prepare a gel in which DNA fragments can be separated based on size
What is the role of protinase K in DNA isolation?
I believe the role of proteinase K in a DNA isolation is just to digest proteins. Proteinase K is a protein digesting enzyme. Digesting proteins is important in a DNA isolation because the proteins included in your DNA before you treat it with proK likely include some DNAses. If you didn't use proK, your DNA would degrade very quickly.
Role of sucrose in DNA isolation from human blood?
Sucrose performs the function of osmoregulation in the protocol of DNA isolation from blood
What is role of Cetyl trimethyl ammonium bromide in DNA isolation?
it act as as a cationic detergent for the isolation dna from the given sample
Role of chloroform in DNA isolation?
It solubilizes lipids and a lot of proteins to remove them from the DNA.
Role of CTAB in DNA isolation?
It sequester carbohydrates in the solution
What is the role of SE-VEG in DNA isolation?
the role seveg in plant DNA extractions is to remove chlorophyll and similar pigments
Role of phenol chloroform in DNA isolation?
phenol is used in order to remove protein impurities from the DNA to yield pure dna while chloroform prevents shearing of DNA during isolation.
What is role of potassum cloride in Tkm1 buffer for DNA isolation?
for cell lysis
What is the role of 2-mercaptoethanol in the isolation of genomic DNA from a plant source?
It is an antioxidant.
Role of RBC lysis solution in DNA isolation?
When isolating DNA from blood, white blood cells (WBC's) are the target. This is because RBC's do not contain a nucleus and therefore do not contain DNA. The function of the lysis buffer is to help in the lysis (or breaking) of white blood cells. WBC's must first be lysed so that the DNA may be released from inside the cell.
What is the role of sorbitol in DNA isolation?
what is the function of sorbitol in producing plant protoplasts
What is the role of sodium acetate in DNA extraction?
sodiom acetat reaction with membrane protein and cause that persipitate and help to dna isolation
Role of mgcl2 in DNA isolation?
mgcl2 work as a cofactor and they enhance the enzymatic reaction..
