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Yes, when you are performing smear preparation, you should always use sterilized water. This will ensure that nothing contaminates your slide.
it makes the organelles of the cells stand out
You can use it for purposes not requiring the water to be sterile.
no either sterile water or sodium chloride 0.9%
Sterile water is used under circumstances where additional pathogens and other microbial contaminants; as found in regular water, need to be avoided.
Yes. Just make sure it is a medical grade sterile water. Don't use boiled tap water. Ideally you should use propylene glycol (PG) as well.
A smear preparation is a techniques that is used as a precursor is many different staining techniques.The smear prep usually calls for the sample bacterium to be placed on a microscope slide (use aseptic technique) with one drop of DI water. Then the slide is allowed to air dry for a few minutes then the slide is passed through a Bunsen burner flame. Use a cloths pin so that you do not burn you hands Only pass the glass microscope slide through the flame not you cloths pin if it is wood it will catch fire. Pass the slide through 3-4 times then you are ready to continue on with your desired staining technique
The smear will not adhere well if there is grease on the slide.
Using sterile water is ideal if that's all you've got to clean your eyes. It's always better to use saline to clean your eyes out, though.
Tap water is used to wash the excess stain from a slide prepared from a smear. You can use tap water instead of distilled water because you aren't worried about a precipitate forming and tap water is much cheaper than distilled.
There is no true substitute for sterile water; though there are many ways to make water sterile. It may be helpful to know what the reason is for the use.... I'd suggest asking your question with more detail. Best wishes.
You are not required to use transfer forceps during a surgical procedure of you are wearing sterile gloves to handle sterile instruments in the sterile field.