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mrna
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What is the best explanation for why a restriction enzyme does not cut the DNA of the cell that produces it?
The cell's DNA does not contain the restriction site.
An enzyme that cuts double-stranded DNA at specific nucleotide sequences?
Such an enzyme is called a restriction endonuclease
What are enzymes cutting DNA at specific sites to form restriction fragments called?
restriction enzymes or endonuclease enzymes
Why ligation can be considered the reverse of the restriction enzyme process?
Restriction enzymes cuts out a specific short nucleotide sequence while as the process of ligation, DNA ligase joins them together. So ligase can be considered the reverse of the restriction enzyme process as it joins DNA fragments together instead of cutting them out.
What is a DNA restriction site?
The restriction site is a sequence of DNA that is recognized by an endonuclease, or a protein that cuts DNA, as a site at which the DNA is to be cut. This cutting happens when restriction enzyme cleaves nucleotides by hydrolyzing the phosphodiester bond between them.
What tool to use when cutting plasmid?
a Restriction Enzyme
What is the best explanation for why a restriction enzyme does not cut the DNA of the cell that produces it?
The cell's DNA does not contain the restriction site.
What is the restriction site of the restriction enzyme Hae III?
The restriction site of Hae III is GGCC. It cuts between the G and the C. This produces blunt ends.
An enzyme that cuts double-stranded DNA at specific nucleotide sequences?
Such an enzyme is called a restriction endonuclease
What are enzymes cutting DNA at specific sites to form restriction fragments called?
restriction enzymes or endonuclease enzymes
Why ligation can be considered the reverse of the restriction enzyme process?
Restriction enzymes cuts out a specific short nucleotide sequence while as the process of ligation, DNA ligase joins them together. So ligase can be considered the reverse of the restriction enzyme process as it joins DNA fragments together instead of cutting them out.
What is a DNA restriction site?
The restriction site is a sequence of DNA that is recognized by an endonuclease, or a protein that cuts DNA, as a site at which the DNA is to be cut. This cutting happens when restriction enzyme cleaves nucleotides by hydrolyzing the phosphodiester bond between them.
What are restriction enzymes?
Restriction enzymes (also known as restriction endonucleases) are proteins which cut DNA up at specific sequences in the genome. For example, the commonly used restriction endonuclease EcoRI recognizes every point in DNA with the sequence GAATTC, and cuts at the point between the Guanine and Adenine. Interestingly, the recognition sequences for most restriction endonucleases are genetic palindromes, e.g., the sequence reads exactly the same backwards on the complementary strand. In the case of EcoRI, the two complementary DNA strands for the recognition sequence are: 5'--GAATTC ---3'3'--CTTAAG--5'
What is the restriction site of the restriction enzyme TaqI?
TaqI's restriction site is:TCGAAGCT
Hi does any one know if I want to put some restriction enzyme close to cDNA should I put some nucleotide befor this restriction enzyme?
Restriction enzyme cuts DNA strand at specific locations Restriction enzyme cuts DNA strand at specific locations
How can bacterium produce restriction enzymes that do not cleave it's DNA?
Restriction enzymes cleave DNA at a particular recognition site -- a particular sequence of nucleotides. You can imagine the following scenarios:1. The bacterial chromosome does not contain the recognition sequence2. The bacterial chromosome contains the recognition sequence, but that particular part of the DNA is either supercoiled to keep the restriction enzyme from finding the sequence, or it's single stranded as when being replicated or transcribed.3. The bacterial chromosome contains the recognition sequence, but that particular part of the DNA is methylated or modified in some other way which prevents the restriction enzyme from attaching.
How do you identify restriction enzyme?
By playing with you.
