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The optimum pH and optimum temperatures of an enzyme are simply levels that the enzyme work most effectively at. These levels contribute to factors such as the enzyme's protein structure. Different levels of pH and temperature can alter the integrity of the protein, this process is called denaturation. When an enzyme undergoes denaturation, its level of activity is inhibited by failure of the substrate to effectively attach itself to the active site.

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15y ago
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15y ago

The optimum enzyme concentration is the point where in the highest velocity is attained. and the highest free enzyme able to interact with the substrate is attained.

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16y ago

how do you calculate turnover number?

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Q: Define total enzyme concentration
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What is the Effect of dilution on enzyme activity?

At a high ion concentration, the ion interfere with the bonds between the side groups of the amino acids making up the enzyme (which is a protein). This causes the enzyme to lose its shape, called denaturation. If the enzyme loses its shape, it can no longer accept and react substrate, so the rate of enzyme activity decreases.


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