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The optimum pH and optimum temperatures of an enzyme are simply levels that the enzyme work most effectively at. These levels contribute to factors such as the enzyme's protein structure. Different levels of pH and temperature can alter the integrity of the protein, this process is called denaturation. When an enzyme undergoes denaturation, its level of activity is inhibited by failure of the substrate to effectively attach itself to the active site.
What else can I help you with?
Taq polymerase enzyme 500 units per ml means how to diluted this enzyme?
To dilute Taq polymerase from 500 units/ml to a desired concentration, calculate the volume of the enzyme needed to achieve the desired units. For example, if you need 100 units, you would dilute 0.2 ml of the 500 units/ml solution in a total volume to reach your desired concentration.
What is Specific activity of protein?
Specific activity of a protein is a measure of its enzymatic activity per unit amount of protein. It is calculated by dividing the amount of enzyme activity by the total protein concentration. Specific activity provides a way to compare enzyme preparations that may contain different amounts of protein.
How do you define the atomic mass of an element?
The total mass of the atom. In other words the total mass of the protons and neutrons of an atom
What one what solutions contains the greatest total ion concentration?
The solution with the greatest total ion concentration will have the highest molarity of ionic compounds dissolved in it. This means it will have the highest concentration of ions in solution. Based on the principles of molarity and solubility, the solution with the most dissolved ionic compounds will have the greatest total ion concentration.
How do you calculate the concentration of the reactant when every reactant decompose completely to form the product?
To calculate the concentration of the product formed when reactants decompose completely, you first need to know the initial concentrations of the reactants and their stoichiometry in the balanced chemical equation. Assuming complete decomposition, the concentration of the product will equal the total moles of the reactants converted into product, divided by the total volume of the solution. Use the formula: [ \text{Concentration of product} = \frac{\text{Total moles of reactants}}{\text{Total volume}} ]. Adjust for any stoichiometric coefficients as necessary based on the balanced equation.
Calculation fraction of enzyme bound to substrate?
The fraction of enzyme bound to substrate can be calculated using the Michaelis-Menten equation: [ES] / [E]t = [S] / (Km + [S]), where [ES] is the concentration of enzyme-substrate complex, [E]t is the total enzyme concentration, [S] is the substrate concentration, and Km is the Michaelis constant. This equation gives the ratio of the concentration of enzyme bound to substrate to the total enzyme concentration at a given substrate concentration.
How do you calculate specific activity of salivary amylase?
Specific activity of salivary amylase can be calculated by dividing the total enzyme activity (in units) by the total protein concentration (in mg). The formula is: Specific activity = Total enzyme activity (units) / Total protein concentration (mg). This calculation gives a measure of the enzyme's activity per unit of protein.
What is the Effect of dilution on enzyme activity?
At a high ion concentration, the ion interfere with the bonds between the side groups of the amino acids making up the enzyme (which is a protein). This causes the enzyme to lose its shape, called denaturation. If the enzyme loses its shape, it can no longer accept and react substrate, so the rate of enzyme activity decreases.
Taq polymerase enzyme 500 units per ml means how to diluted this enzyme?
To dilute Taq polymerase from 500 units/ml to a desired concentration, calculate the volume of the enzyme needed to achieve the desired units. For example, if you need 100 units, you would dilute 0.2 ml of the 500 units/ml solution in a total volume to reach your desired concentration.
What is Specific activity of protein?
Specific activity of a protein is a measure of its enzymatic activity per unit amount of protein. It is calculated by dividing the amount of enzyme activity by the total protein concentration. Specific activity provides a way to compare enzyme preparations that may contain different amounts of protein.
Define total product?
It is a product that is total
What amounts do you compare when measureing concentration?
To measure concentration, you compare the amount of the solute to the total amount of the solution.
Define Total fertility rate?
total fertility is where women slash girls are having kids/babies.
What term can be define as a government with a king or queen that has total power?
monarchy
How many people were imprisoned at the Jewish concentration camps?
Their was no Jewish Concentration Camp but in total between 15-16 Million Jews were sent to Nazi Concentration Camps.
How do you define the atomic mass of an element?
The total mass of the atom. In other words the total mass of the protons and neutrons of an atom
How many people died in 1933 in the concentration camp Dachau?
It was actually Dachau Concentration Camp, and a total of 31,951 deaths. 25,334 of them Jews.
