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The optimum pH and optimum temperatures of an enzyme are simply levels that the enzyme work most effectively at. These levels contribute to factors such as the enzyme's protein structure. Different levels of pH and temperature can alter the integrity of the protein, this process is called denaturation. When an enzyme undergoes denaturation, its level of activity is inhibited by failure of the substrate to effectively attach itself to the active site.
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Taq polymerase enzyme 500 units per ml means how to diluted this enzyme?
To dilute Taq polymerase from 500 units/ml to a desired concentration, calculate the volume of the enzyme needed to achieve the desired units. For example, if you need 100 units, you would dilute 0.2 ml of the 500 units/ml solution in a total volume to reach your desired concentration.
What is Specific activity of protein?
Specific activity of a protein is a measure of its enzymatic activity per unit amount of protein. It is calculated by dividing the amount of enzyme activity by the total protein concentration. Specific activity provides a way to compare enzyme preparations that may contain different amounts of protein.
How do you define the atomic mass of an element?
The total mass of the atom. In other words the total mass of the protons and neutrons of an atom
What one what solutions contains the greatest total ion concentration?
The solution with the greatest total ion concentration will have the highest molarity of ionic compounds dissolved in it. This means it will have the highest concentration of ions in solution. Based on the principles of molarity and solubility, the solution with the most dissolved ionic compounds will have the greatest total ion concentration.
What is the concentration in mm percent of a solution prepared from 50.0g Na-Cl and 150.0g of water?
To find the concentration in mass percent, first calculate the total mass of the solution (50.0g NaCl + 150.0g water = 200.0g total). Then, divide the mass of NaCl by the total mass of the solution and multiply by 100 to get the concentration in mass percent: (50.0g NaCl / 200.0g total) * 100 = 25.0% NaCl.
Calculation fraction of enzyme bound to substrate?
The fraction of enzyme bound to substrate can be calculated using the Michaelis-Menten equation: [ES] / [E]t = [S] / (Km + [S]), where [ES] is the concentration of enzyme-substrate complex, [E]t is the total enzyme concentration, [S] is the substrate concentration, and Km is the Michaelis constant. This equation gives the ratio of the concentration of enzyme bound to substrate to the total enzyme concentration at a given substrate concentration.
How do you calculate specific activity of salivary amylase?
Specific activity of salivary amylase can be calculated by dividing the total enzyme activity (in units) by the total protein concentration (in mg). The formula is: Specific activity = Total enzyme activity (units) / Total protein concentration (mg). This calculation gives a measure of the enzyme's activity per unit of protein.
What is the Effect of dilution on enzyme activity?
At a high ion concentration, the ion interfere with the bonds between the side groups of the amino acids making up the enzyme (which is a protein). This causes the enzyme to lose its shape, called denaturation. If the enzyme loses its shape, it can no longer accept and react substrate, so the rate of enzyme activity decreases.
Taq polymerase enzyme 500 units per ml means how to diluted this enzyme?
To dilute Taq polymerase from 500 units/ml to a desired concentration, calculate the volume of the enzyme needed to achieve the desired units. For example, if you need 100 units, you would dilute 0.2 ml of the 500 units/ml solution in a total volume to reach your desired concentration.
Define total product?
It is a product that is total
What is Specific activity of protein?
Specific activity of a protein is a measure of its enzymatic activity per unit amount of protein. It is calculated by dividing the amount of enzyme activity by the total protein concentration. Specific activity provides a way to compare enzyme preparations that may contain different amounts of protein.
What amounts do you compare when measureing concentration?
To measure concentration, you compare the amount of the solute to the total amount of the solution.
Define Total fertility rate?
total fertility is where women slash girls are having kids/babies.
What term can be define as a government with a king or queen that has total power?
monarchy
How many people were imprisoned at the Jewish concentration camps?
Their was no Jewish Concentration Camp but in total between 15-16 Million Jews were sent to Nazi Concentration Camps.
How do you define the atomic mass of an element?
The total mass of the atom. In other words the total mass of the protons and neutrons of an atom
How many people died in 1933 in the concentration camp Dachau?
It was actually Dachau Concentration Camp, and a total of 31,951 deaths. 25,334 of them Jews.
