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What is the purpose of sodium azide in the Bile Esculin test?
The purpose of the bile esculen azide agar is to inhibit the growth of gram-positive organisms.
Does lactococcus lactis grow in bile esculin?
No it doesn't. Entercocci and members of the streptococcus group can.
Role of bile salt in macconkey agar?
it inhibits the growth of Gram positive bacteria
What is the indicator that turns the agar medium pink with copper?
MacConkey agar contains bile salts, lactose, peptone. crystal violet dye, and neutral red dye. The agar appears a medium pink with a copper undertone.
Does micrococcus luteus grow on macconkey agar?
MacConkey agar is designed to grow Gram-negative bacteria and also contains crystal violet dye which inhibits the growth of Gram-positive bacteria. Since S. aureus is Gram-positive it should not grow on MacConkey agar. No. MacConkey agar inhibits MOST of the Gram-positive bacteria. There are some Gram-positive bacteria that can tolerate bile-salt are able to grow in MacConkey agar. Enterococcus and some species of Staphylococcus are able to grow in MacConkey. (lack citation though)
What is the purpose of sodium azide in the Bile Esculin test?
The purpose of the bile esculen azide agar is to inhibit the growth of gram-positive organisms.
Does lactococcus lactis grow in bile esculin?
No it doesn't. Entercocci and members of the streptococcus group can.
What is the fucntion of oxgall?
Oxbile (Oxgall) is dehydrated bile for use in preparing microbiological culture media. Oxbile is manufactured from large quantities of fresh bile by rapid evaporation of the water content. Bile is composed of fatty acids, bile acids, inorganic salts, sulfates, bile pigments, cholesterol, mucin, lecithin, glycuronicacids, porphyrins, and urea. The use of Oxbile insures a regular supply of bile, and uniformity impossible to obtain with fresh materials. Oxbile is dehydrated fresh bile and prepared specifically for differentiation of bile tolerant microorganisms. A 10% solution of dehydrated bile is equivalent to a fresh bile solution. It is usually incorporated into media e.g., Bile Esculin Agar and Brilliant Green Bile Agar, used for the determination of enteric pathogens. Oxbile is also found in Littman Agar, a selective fungal medium. It is used as a selective agent for the isolation of Gram-negative microorganisms, inhibiting Gram-positive bacteria. The major composition of Oxbile is taurocholic and glycocholic acids.
What is the function on bile in Eosin Methylene Blue agar?
To make the Eosin Methylene BLue Agar look cool and hip.
Role of bile salt in macconkey agar?
it inhibits the growth of Gram positive bacteria
Why coliform show pink color colony in violet red bile agar?
Violet Red Bile Agar is recommended for use in the microbiological analysis of milk and other dairy products and for use in the examination of water. Violet Red Bile Agar is a selective medium which detects the growth of lactose fermenting coliforms. Coliforms colonies lower the pH of the medium subsequently causing their colonies to look red (neutral red dye) and to precipitate the bile salts. Crystal violet and bile salts inhibit the growth of Gram positive microorganisms.
What is the indicator that turns the agar medium pink with copper?
MacConkey agar contains bile salts, lactose, peptone. crystal violet dye, and neutral red dye. The agar appears a medium pink with a copper undertone.
What is macconkey agar made out of?
Macconkey agar consists of agar, lactose, peptone, neutral red dye, crystal violet dye & bile salts. Bile salts inhibit growth of Gram positive bacteria & allow growth of Gram negative bacteria only. It differentiates lactose fermentating Gram negative bacteria from non lactose fermentating Gram negative bacteria.
Does micrococcus luteus grow on macconkey agar?
MacConkey agar is designed to grow Gram-negative bacteria and also contains crystal violet dye which inhibits the growth of Gram-positive bacteria. Since S. aureus is Gram-positive it should not grow on MacConkey agar. No. MacConkey agar inhibits MOST of the Gram-positive bacteria. There are some Gram-positive bacteria that can tolerate bile-salt are able to grow in MacConkey agar. Enterococcus and some species of Staphylococcus are able to grow in MacConkey. (lack citation though)
What is Mcconkey agar media?
It's a differential agar used to differentiate between lactose fermenters and non-lactose fermenteing bacteria. Chemical composition of Mac Conkey agar Peptone 20.0g Trehalose 10.0g Bile salts(Difco) 1.5g Sodium Cloride 5.0 Neutral Red 0.05g Crystal Violet 0.001 Agar 15.0g Distelled water 1.0 liter pH: 7.4±0.2 (25 °C)
Why most of the gram positive bacteria cannot grow on MacConkey Agar?
gram positive bacteria cannot grow due to the presence of bile salts and crystal violet in maConkey media.
Is a bile duct the same as a bile?
no because bile is stored in the bile duct
