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What is e-value of a optical brightening agent?
e-value of an optical brightening agent is the absorbance value obtained (in the UV region), when the absorbance of 1% solution is measured using a cuvette of 1 cm path length. T.J.R.PILLAI
What does Wavelenght vs absorbance graph depicts in u-v spectroscopy?
A wavelength vs absorbance graph depicts in uv spectroscopy shows the different colored wavelenths of UV light and how they are absorbed and percieved, and which ones are visible and which ones are not.
Why does diethyl ether not have uv spectrum?
The UV absorbance over 190 nm is not significant because diethyl ether hasn't aromatic rings..
Can a turbid sample be measured for absorbance in UV spectrophotometer?
Yes. Olis, Inc. (Bogart, GA) offers the CLARiTY line of spectrophotometers, which are specifically designed to give accurate absorbance readings in highly turbid samples.
How to calculate lambda max?
using uv-visible spectrophotometer concentration vs absorbance is plotted and the maximum absorbance of the drug is lambda max of the drug. then after it will decrease. still if needed clarification, refer beer lambert"s law
What is the definition of cut off you uv-visible spectrophotometer?
UV cut-off is the wavelength at which the solvent absorbance in a 1 cm path length cell is equal to 1 AU (absorbance unit) using water in the reference cell. ( © 2000, LC Resources Inc.)
What is e-value of a optical brightening agent?
e-value of an optical brightening agent is the absorbance value obtained (in the UV region), when the absorbance of 1% solution is measured using a cuvette of 1 cm path length. T.J.R.PILLAI
What does Wavelenght vs absorbance graph depicts in u-v spectroscopy?
A wavelength vs absorbance graph depicts in uv spectroscopy shows the different colored wavelenths of UV light and how they are absorbed and percieved, and which ones are visible and which ones are not.
Why does diethyl ether not have uv spectrum?
The UV absorbance over 190 nm is not significant because diethyl ether hasn't aromatic rings..
What is effect of solvent in uv spectroscopy?
effect of solvent on UV-Visible spectrum
What is the relation of Beers Lambert's law in UV Visible spectrometer?
The Beer-Lambert law Absorbance = (extinction coefficent)(pathlength of light)(concentration) allows you to measure the absorbance of sample in a UV spec, and change the rate from absorbance units / time to change in concentration / time. the pathlength of light being the width of the cuvette and the extinctin coefficent being specific to the product molecule.
What is hyperchromic and hypochromic effect?
In reference to the melting of DNA: as DNA melts (denatures from a double-stranded molecule to two single strands) the UV absorbance INCREASES. This absorbance increase is referred to as a "hyperchromic shift" or the hyperchromic effect. Thinking about this situation in reverse: the UV absorbance DECREASES as two DNA strands anneal to form double stranded DNA. This is referred to as the "hypochromic effect". (Please note, there is an answer on answers.com that incorrectly states the opposite, that absorbance decreases with melting. This is incorrect. Two single strands of DNA have higher absorbance than the double-stranded molecule.)
Can a turbid sample be measured for absorbance in UV spectrophotometer?
Yes. Olis, Inc. (Bogart, GA) offers the CLARiTY line of spectrophotometers, which are specifically designed to give accurate absorbance readings in highly turbid samples.
How to calculate lambda max?
using uv-visible spectrophotometer concentration vs absorbance is plotted and the maximum absorbance of the drug is lambda max of the drug. then after it will decrease. still if needed clarification, refer beer lambert"s law
Why Potassium dichromate is used for calibrate the control of absorbance of UV spectrophotometer?
Potassium dichromate is used as the primary standard for UV spectrophotometry because of its properties. It is pure, stable, has no waters of hydration, and has a high molar mass.
What is the unit of absorbance?
Mol-1cm-1
Is wavelength or absorbance the dependent variable?
"absorbance"Since in the experiment, you probably choose the wavelength, then measure the absorbance (absorption?, the absorbance is the dependent variable.
