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Explain fully the role of naoh in the lysis procedure to obtain covalently closed plasmid molecules?
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Role of NaOH in plasmid DNA isolation?
Plasmid isolation involves growing the plasmid under conditions that are suitable for genes to come into play. For example the gene for ampicillin resistance; the bacteria with plasmids are placed with ampicillin so their genes can be seen for those who survived. Sodium hydroxide acts a detergent in the extraction process. A detergent's main role is to break down cell walls and cell membranes. How so? They act as poking holes into membranes. However, for the isolation of plasmid, the NaOH acts
Role of phenol chloroform isoamyl alcohol in plasmid DNA extraction?
The actual role of phenol chloroform isoamyl alcohol in a plasmid DNA extraction is to purify the DNA. The alcohol will act in part as a detergent.
Which enzyme should she use to join the sticky ends of the gene and the plasmid?
chips
Why you use LiCl in plasmid isolation by telt method?
helps in DNA ppt
A plasmid that contains a gene for human growth hormone is and example of what?
recombinant dna
Molecules of extrachromosomal DNA are also known as?
lysosomes
What is recombinant Plasmid?
A plasmid is a small, circular, double-stranded DNA molecule that is distinct from a cell's chromosomal DNA. ... Researchers can insert DNA fragments or genes into a plasmid vector, creating a so-called recombinant plasmid. This plasmid can be introduced into a bacterium by way of the process called transformation.
What is the rule of plasmid in biotechnology?
Plasmids are double stranded DNA molecules that serve as vector to carry a foreign gene in to the host.
Bacteria can take up foreign DNA molecules packaged in plasmids?
yes. Some bacteria can take up plasmids present in the solution around them or by getting the plasmid from another bacteria which possess a special "fertility" F-plasmid.
When is a plasmid considered a recombinant plasmid?
When the original function of the gene in the plasmid is altered or another gene is inserted in the non- coding region of the plasmid is called the recombinant plasmid.
What is the function of the Ti plasmid?
what is the function of the plasmid
Which is the plasmid that increases resistance to antibiotics?
R-plasmid
What plasmid that can degrade toluic acid?
TOL plasmid
The restriction enzyme used in constructing hybrid molecules of certain gene sequences and plasmid DNA acts by?
opening DNA molecules at specific sites,leaving sticky ends exposed. find me on kik one1992
Analogy for plasmid?
F-plasmid (fertility plasmid) of any conjugative bateria or Ti-plasmid (tumor-inducing) of Agrobacterium tumaefaciens.
A plasmid is a type of?
Plasmid is extrachromosomal DNA capable of self replication.
What is a self-transmissible plasmid?
A plasmid which encodes genes for its own transfer.
