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Explain the chemical mechanism for detecting indole in a bacterial culture?
Wiki User
∙ 15y ago
Indole is extracted from the medium and into the reagent layer (Kovac's) by the acidified butyl alcohol component and forms a complex with the p-dimethylaminobenzaldehyde, yielding the cherry red color seen when the culture is indole positive.
Wiki User
∙ 15y ago
Indole can be detected in a bacterial culture by using Kovac's reagent, which reacts with indole to produce a red color. The chemical mechanism involves the reaction between the indole and the reagent, resulting in the formation of a red complex that can be visually observed. This test is commonly used in microbiology to differentiate between bacteria that can produce indole (positive result) and those that cannot (negative result).
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