Glucose is added to increase the osmotic pressure outside the cells.glucose should also be added to maintain osmolarity and prevent the buffer from bursting the cells.
maintain the osmatic pressure
Isolation of a plasmid from a bacterium
When the original function of the gene in the plasmid is altered or another gene is inserted in the non- coding region of the plasmid is called the recombinant plasmid.
Perhaps you mean a restriction enzyme, but not disrupting the function of whatever is not too clear. I think if you cut a plasmid with any restriction enzyme I am familiar with the function of that plasmid would be disrupted.
Plasmid isolation has a step called washing step that carried out in the column in which the plasmid DNA are already bind. There are two wash solution, first one endo wash buffer that wash the traces of bacterial membrane remnants such as LPS. Wash buffer two has ethanol wash off any protein contaminants present on the column. These wash steps ensure the purify of isolated plasmid DNA.
Ti plasmid functions to induce turmor or a desease known as "crown gall" to the most dicot (rarely monocot) plants. Transfer DNA or T-DNA will be released during the infection process into the plant cell and integrate with the DNA host. Hence, the plant host is already infected. That's the important function of the Ti plasmid, if there are no such plasmid exist, then the agrobacterium lost its pathogenic function.
removes the remaining protein which is left after denaturation
it helps in the removal of proteins from nucleic acid
Isolation of a plasmid from a bacterium
what is the function of the plasmid
When the original function of the gene in the plasmid is altered or another gene is inserted in the non- coding region of the plasmid is called the recombinant plasmid.
helps in DNA ppt
to neutralise the alkaline conditions.
Perhaps you mean a restriction enzyme, but not disrupting the function of whatever is not too clear. I think if you cut a plasmid with any restriction enzyme I am familiar with the function of that plasmid would be disrupted.
Plasmid isolation has a step called washing step that carried out in the column in which the plasmid DNA are already bind. There are two wash solution, first one endo wash buffer that wash the traces of bacterial membrane remnants such as LPS. Wash buffer two has ethanol wash off any protein contaminants present on the column. These wash steps ensure the purify of isolated plasmid DNA.
In plasmid isolation RNA behaves as an unwanted material so to separate it out RNAase is required which breaks down the RNA. This is done to get pure quality of the product.
Plasmids have small pockets of DNA in them.
Cold ethanol or isopropanol is used to precipitate the plasmid DNA, DNA is insoluble in alcohol and clumps or clings together. Centrifuging will cause the precipitate to form a pellet which can be decanted from the unwanted supernatant. Where as if compared with RNA isolation isopropanol is less efficient in precipitating RNA, where in presence of Lithium chloride or ammonium ions can give a good yield