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The steps in Gram staining are:
1. crystal violet added to the smear
2. iodine, the mordant (this fixes the violet)
3. a decolorizer made of acetone and alcohol
4. safranin, the counterstain
If the cell is Gram +, the decolorizer can not remove the violet. If it is Gram -, the decolorizer can remove the violet and the cell can be then colored with the dye, safranin.
Bacteria are grouped in 4 groups by Gram stain:
Gram-positive, the cell wall retains crystal Violet.
Gram-negative, the cell wall does not retain crystal Violet.
Graham not reactive, no staining whatsoever.
Graham variable, uneven staining.
What else can I help you with?
How we can use acetocarmine to stain the onion root tip cells?
To stain onion root tip cells with acetocarmine, first immerse the root tip in acetocarmine solution for a few minutes to allow the stain to penetrate the cells. Then, rinse the root tip with water to remove excess stain while retaining the stained cells. Mount the root tip onto a slide with a drop of water and cover with a coverslip for observation under a microscope.
Which organ of human body has the highest blood supply?
By weight probably the brain, but the liver, kidneys and heart are close seconds. Per 100 gm of tissue: Carotid Bodies: 2000 ml/min/100 gm tissue Kidneys: 360 ml/min/100 gm tissue Adrenal glands: 300 ml/min/100 gm tissue Liver: 95 ml/min/100 gm tissue Heart: 70 ml/min/100 gm tissue Brain: 50 ml/min/100 gm tissue
How many times does a bacteria cell reproduce in a day?
The rate at which a bacteria cell reproduces can vary depending on the species and environmental conditions. Some bacteria can replicate every 20 minutes under optimal conditions, while others may have longer replication times. In general, bacteria can reproduce multiple times in a day if conditions are favorable.
What do the two men at the Rock of the Falling Flower want from Tree-ear?
i think that they want flower pot
What is the composition of PEA agar?
Phenylethyl Alcohol Agar (PEA) is prepared using the following incredients:Pancreatic Enzyme digest of Casein.......15.0gPapaic digest of Soybean Meal.................5.0gSodium Chloride.....................................5.0gYeast Extract..........................................5.0gPhenylethanol........................................2.5gVitamin K..........................................10.0mgHemin.................................................5.0mgL-Cystine.............................................0.4mgSheep Blood........................................50.0mlAgar.....................................................15.0gComponents are mixed in 1 L water. If making less than 1 L media, change components accordingly. Media should be prepared at pH 7.3 +/- 0.2. After mixing components and adjusting pH, autoclave at 121 degrees C for 20 min then placed into water bath at 50 degrees C to allow it to cool enough to be poured.
How can you differentiate gram positive and gram negative bacteria?
The process of gram staining is simple. 1)smear bacteria from pure culture onto slide, heat fix 2)flood with crystal violet (1min) 3)Add iodine (1 min) 4)acid/alcohol wash (1 min) 5)Flood with safranine (1min) 6)Air dry and examine. These times are for clinical microbiology and experimental methods employ optimal and more precise times (but overall its pretty close). Down side of this method is that you must smear bacteria onto the slide and fix it by heating the underside of the slide with a bunsen burner. if they are pink then you have gram negative (Gram's stain didnt stick) if its purple then its gram positive(Gram's stain did stick) This is due to the peptidoglycan layers. Gram negative bacteria have only a thin layer of peptidoglycan as part of the cell membrane/wall where Gram positive have a very think peptidoglycan layer. Source(s): Medical Microbiology
How do scientists distinguish between gram-positive and gram-negative bacteria?
The process of gram staining is simple. 1)smear bacteria from pure culture onto slide, heat fix 2)flood with crystal violet (1min) 3)Add iodine (1 min) 4)acid/alcohol wash (1 min) 5)Flood with safranine (1min) 6)Air dry and examine. These times are for clinical microbiology and experimental methods employ optimal and more precise times (but overall its pretty close). Down side of this method is that you must smear bacteria onto the slide and fix it by heating the underside of the slide with a bunsen burner. if they are pink then you have gram negative (Gram's stain didnt stick) if its purple then its gram positive(Gram's stain did stick) This is due to the peptidoglycan layers. Gram negative bacteria have only a thin layer of peptidoglycan as part of the cell membrane/wall where Gram positive have a very think peptidoglycan layer. Source(s): Medical Microbiology
How do scientists distinguish between Gram- positive and Gram-negative bacteria?
The process of gram staining is simple. 1)smear bacteria from pure culture onto slide, heat fix 2)flood with crystal violet (1min) 3)Add iodine (1 min) 4)acid/alcohol wash (1 min) 5)Flood with safranine (1min) 6)Air dry and examine. These times are for clinical microbiology and experimental methods employ optimal and more precise times (but overall its pretty close). Down side of this method is that you must smear bacteria onto the slide and fix it by heating the underside of the slide with a bunsen burner. if they are pink then you have gram negative (Gram's stain didnt stick) if its purple then its gram positive(Gram's stain did stick) This is due to the peptidoglycan layers. Gram negative bacteria have only a thin layer of peptidoglycan as part of the cell membrane/wall where Gram positive have a very think peptidoglycan layer. Source(s): Medical Microbiology
Can you use min wax oil based stain on concrete?
No, it is intended for wood and soaks in too much to look good on concrete. HD sells a good variety of concrete stain.
What is the technique for gram staining test?
the gram staining procedure involves four basic steps; 1.the smear is first flooded wit the primary stain crystal violet dye.in this case the primary stain is the first dye applied in any multicelled staining procedure and it stains all the cells. 2.the smear is rinsed with water to remove any excess crystal violet and then its flooded wit a dilute solution of iodine called grams iodine.iodine acts as a mordant.i.e. the substance that increases the interaction and affinity of cellular components for a dye.this is done so that the cells can stain more strongly. in this case the iodine complex thereby decreases the solubility of the dye within the cells 3.the stained smear is rinsed again and then 95% alcohol or a mixture of alcohol and acetol is briefly added.this solvent acts as decolorizing agents and they readily remove the dye iodine complex from gram negative but not from gram positive bacteria. 4.a counter stain is then applied to import a contrasting color to the now colorless gram negative bacteria.for this purpose,the red dye safrannin is used.this dye stains gram negative as well as gram positive bacteria but because the gram positive are already stained purple,it impacts little difference
Which basic dyes were used to stain the smears?
1 Gram stainDeveloped by the Danish physician"Hans Christian Gram"Most commonly employed.Important of all the diagnostic staining techniques.By this method bacteria can be recognised as Gram-positive (blue-black) if they retain the primary dye complex of methyl violet and iodine in the face of attempted decolourization or as Gram-negative (red) if decolourisation occurs as shown by the cell accepting the counter stain.Procedure:Flood slide with crystal -10 seconds.washFlood with Gram's iodine - 10 seconds.washCarefully decolorize with 95% ethanolwashFlood with safranin (pink color) - 10 seconds.washAir dry,2 ZEIHL-NEELSEN'S METHOD (Acid-Fast Stain)ACID FAST Os Contains waxlike lipoidal material affecting staining quality.•Carbolfuchsin is primary stain.•Acid fast organisms resist decolorization with acid alcohol.•After decolorization, methyelene blue is added to organisms to counterstain any material that is not acid fast.•It is used for examination of Mycobacterium.•The principle of staining is depend on the resistance of this type of bacteria to decolarization by acid alcohol, because the cell wall contain waxy material (mycolic acid) which prevent the removal of carbol fuchsin from the cell.Staining ProcedureFlood the slide with strong carbol fuchsin and heat until steam rises (but do not boil).After 3-4 min apply more heat until rises again; do not let the stain dry on the slide.About 5-7 min after the first application of heat washes the slide thoroughly under running water.Decolourize in acid-alcohol until all traces of red have disappeared from the film. Decolourization should not be attempted in one stage; there should be intermittent washings in water and re-application of acid-alcohol.Wash well with water and counter stain with Methylene blue for 1 min.Wash and stand end to drain.Acid-fast organisms are red, other organisms are blue.
How do you remove jeyes fluid stain from my white bathtub?
I Used some bleach let it sit for 5 min on the stain, and then used a bit of boiling water and scrubbed with a sponge and it was gone
Remove stain from rayon fabric?
To remove a stain from rayon fabric, start by blotting the stain with a cloth soaked in cold water and a mild detergent. Avoid rubbing the stain, as it can set it further. If the stain persists, consider taking the garment to a professional cleaner.
How do you change the head light time delay to 3 min?
how do you change the head light time delay to 3 min. how to i get into the DIC information center, what steps do i take! (DIC)
Trace out the algorithm Max Min on a data set containing atleast 8 elements?
Steps to perform MaxMin on a data set (2,4,6,3,8,1,9,7) are:(2,4,6,3) (8,1,9,7)((2,4)(6,3)) ((8,1)(9,7))In sublist (4,6), max is 6 and min is 4. In sublist (8,9), max is 9 and min is 8.Comparing max and min values of sublist (2,4) and sublist (6,3), value of max is 6 and min is 2.Therefore, for sublist (2,4,6,3) max is 6 and min is 2.Similarly, comparing max and min values of sublist (8,1) and sublist (9,7), value of max is 9 and min is 1.Therefore, for sublist (8,1,9,7) max is 9 and min is 1.Finally, comparing max and min values of sublist (2,4,6,3) and sublist (8,1,9,7), value of max is 9 and min is 1. Steps to perform MaxMin on a data set (2,4,6,3,8,1,9,7) are:(2,4,6,3) (8,1,9,7)((2,4)(6,3)) ((8,1)(9,7))In sublist (4,6), max is 6 and min is 4. In sublist (8,9), max is 9 and min is 8.Comparing max and min values of sublist (2,4) and sublist (6,3), value of max is 6 and min is 2.Therefore, for sublist (2,4,6,3) max is 6 and min is 2.Similarly, comparing max and min values of sublist (8,1) and sublist (9,7), value of max is 9 and min is 1.Therefore, for sublist (8,1,9,7) max is 9 and min is 1.Finally, comparing max and min values of sublist (2,4,6,3) and sublist (8,1,9,7), value of max is 9 and min is 1. Steps to perform MaxMin on a data set (2,4,6,3,8,1,9,7) are:(2,4,6,3) (8,1,9,7)((2,4)(6,3)) ((8,1)(9,7))In sublist (4,6), max is 6 and min is 4. In sublist (8,9), max is 9 and min is 8.Comparing max and min values of sublist (2,4) and sublist (6,3), value of max is 6 and min is 2.Therefore, for sublist (2,4,6,3) max is 6 and min is 2.Similarly, comparing max and min values of sublist (8,1) and sublist (9,7), value of max is 9 and min is 1.Therefore, for sublist (8,1,9,7) max is 9 and min is 1.Finally, comparing max and min values of sublist (2,4,6,3) and sublist (8,1,9,7), value of max is 9 and min is 1. sonika aggarwal GNIIT
How do you remove phenazopyridine stains from wool garment?
Soak stain with hydrogen peroxide (yes the same stuff used to disinfect a cut) for about 3 minutes. Make a paste from liquid laundry detergent and baking soda and apply it to the stain. scrub the stain and apply white vinegar. let the solution bubble. Rinse the stain with hot water and let soak in a solution of baking soda and Luke warm tap water for 15 min. rinse with Luke warm tap water and was as usual
How we can use acetocarmine to stain the onion root tip cells?
To stain onion root tip cells with acetocarmine, first immerse the root tip in acetocarmine solution for a few minutes to allow the stain to penetrate the cells. Then, rinse the root tip with water to remove excess stain while retaining the stained cells. Mount the root tip onto a slide with a drop of water and cover with a coverslip for observation under a microscope.
